Changes in chemical and ultrastructural composition of ameroid constrictors following in vitro expansion

Anderson, Thomas S.; Rance, Graham A.; Jiang, Long; Piggott, Matthew J.; Field, Elinor J.; Chanoit, Guillaume P.

Abstract

Objective
To (1) characterise the chemical and ultra-structural composition of ameroid constrictors, at a native state and during in vitro expansion and (2) determine the presence of irritant compounds at the surface or within the bulk of the constrictor.

Methods
Twelve sterile, commercially packaged ameroid constrictors (3 repeats of 3.5 mm, 5 mm, 6 mm and 7 mm internal diameter) were analysed by time-of-flight secondary ion mass spectrometry, Raman spectroscopy, attenuated total reflectance Fourier transform infrared spectroscopy and scanning electron microscopy.

Results
Ameroid constrictors have a composition commensurate with casein with little-to-no intra- or inter- constrictor variation. Microscopic analysis indicated that the topographical features of the constrictor surfaces were consistent between all constrictors. Following in vitro expansion there was a reproducible decrease in Ca+ ion content, little-to-no variation in secondary protein structure and morphological changes including the presence of surface aggregates present only at the inner surface of the ameroid constrictor. The potential irritant polydimethylsiloxane was found on the constrictor surface. A trace quantity of an ion fragment assigned as formaldehyde was detected; however, the extremely low level is thought highly unlikely to play a role as an inflammatory trigger clinically.

Discussion
There is a high degree of inter- and intra-constrictor homogeneity from different batches, and reproducible ultrastructural changes following in vitro expansion. Variations occur in both the surface chemistry and topography of the device during closure, which can potentially affect the biomaterial-host interface. Ameroid constrictor closure mechanism is likely involving calcium-mediated inter-protein interactions rather than the imbibition of water only.

Citation

Anderson, T. S., Rance, G. A., Jiang, L., Piggott, M. J., Field, E. J., & Chanoit, G. P. (2018). Changes in chemical and ultrastructural composition of ameroid constrictors following in vitro expansion. PLoS ONE, 13(11), 1-13. https://doi.org/10.1371/journal.pone.0207471

• Journal Article Type: Article
• Acceptance Date: Oct 31, 2018
• Online Publication Date: Nov 15, 2018
• Publication Date: Nov 15, 2018
• Deposit Date: Sep 13, 2019
• Publicly Available Date: Sep 16, 2019
• Journal: PLOS ONE
• Electronic ISSN: 1932-6203
• Publisher: Public Library of Science
• Peer Reviewed: Peer Reviewed
• Volume: 13
• Issue: 11
• Article Number: e0207471
• Pages: 1-13
• DOI: https://doi.org/10.1371/journal.pone.0207471
• Public URL: https://nottingham-repository.worktribe.com/output/1874470
• Publisher URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0207471
• Contract Date: Sep 13, 2019