Surface-sampling mass spectrometry to study proteins and protein complexes

Wong, Kei F. Carver; Greatorex, Rebecca E.; Gidman, Charlotte E.; Rahman, Sidrah; Griffiths, Rian L.

Abstract

This review aims to summarise the current capabilities of surface mass spectrometry (MS) approaches that offer intact protein analysis, and that of non-covalent complexes. Protein analysis is largely achieved via matrix-assisted laser desorption/ionisation (MALDI), which is in itself a surface analysis approach or solvent-based electrospray ionisation (ESI). Several surface sampling approaches have been developed based on ESI, and those that have been used for intact protein analysis will be discussed below. The extent of protein coverage, top-down elucidation, and probing of protein structure for native proteins and non-covalent complexes will be discussed for each approach. Strategies for improving protein analysis, ranging from sample preparation, and sampling methods to instrument modifications and the inclusion of ion mobility separation in the workflow will also be discussed. The relative benefits and drawbacks of each approach will be summarised, providing an overview of current capabilities.

Citation

Wong, K., Greatorex, R., Gidman, C., Rahman, S., & Griffiths, R. (2023). Surface-sampling mass spectrometry to study proteins and protein complexes. Essays in Biochemistry, https://doi.org/10.1042/ebc20220191

• Journal Article Type: Article
• Acceptance Date: Jan 5, 2023
• Online Publication Date: Feb 7, 2023
• Publication Date: Feb 7, 2023
• Deposit Date: Mar 10, 2023
• Publicly Available Date: Mar 13, 2023
• Journal: Essays in Biochemistry
• Print ISSN: 0071-1365
• Electronic ISSN: 1744-1358
• Publisher: Portland Press
• Peer Reviewed: Peer Reviewed
• DOI: https://doi.org/10.1042/ebc20220191
• Keywords: imaging techniques, mass spectrometry, proteomics, surface analysis
• Public URL: https://nottingham-repository.worktribe.com/output/17088265
• Publisher URL: https://portlandpress.com/essaysbiochem/article/doi/10.1042/EBC20220191/232538/Surface-sampling-mass-spectrometry-to-study