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Use of biologically active 3D matrix for extensive skin defect treatment in veterinary practice: case report

Zakirova, Elena Yu; Shalimov, Dmitry V.; Garanina, Ekaterina E.; Zhuravleva, Margarita N.; Rutland, Catrin S.; Rizvanov, Albert A.


Elena Yu Zakirova

Dmitry V. Shalimov

Ekaterina E. Garanina

Margarita N. Zhuravleva

Albert A. Rizvanov


Objectives: Large full-thickness skin defects represent a serious veterinary problem.
Methods: We have developed novel bioactive 3D-matrixes based on fibrin glue Tissucol (Baxter), containing the combination of the adenoviral constructs with genes vascular endothelial growth factor 165 (VEGF165) and fibroblast growth factor two (FGF2; construct Ad5-VEGF165 + Ad5-FGF2) or multipotent mesenchymal stem cells, genetically modified with these constructs.
Results: In vitro studies confirmed the biosynthesis of VEGF165 and FGF2 mRNA in the transduced cells. Ad5-VEGF165 + Ad5-FGF2- transduced multipotent mesenchymal stem cells showed an enhanced capacity to form capillary-like tubes in vitro. Bioactive 3D-matrix application enhanced granulation tissue formation and epithelialization of non-healing, large bite wounds in a dog. Successful wound healing was observed with a positive clinical outcome for the canine patient. This research and application of regenerative gene therapy alongside a novel bioactive 3D-matrix shows promising clinical applications for the future in both dogs and other mammals including humans.


Zakirova, E. Y., Shalimov, D. V., Garanina, E. E., Zhuravleva, M. N., Rutland, C. S., & Rizvanov, A. A. (2019). Use of biologically active 3D matrix for extensive skin defect treatment in veterinary practice: case report. Frontiers in Veterinary Science, 6,

Journal Article Type Article
Acceptance Date Feb 21, 2019
Online Publication Date Mar 15, 2019
Publication Date Mar 15, 2019
Deposit Date Mar 18, 2019
Publicly Available Date Mar 18, 2019
Journal Frontiers in Veterinary Science
Print ISSN 2297-1769
Electronic ISSN 2297-1769
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 6
Article Number 76
Public URL
Publisher URL
Additional Information Use of Biologically Active 3D Matrix for Extensive Skin Defect Treatment in Veterinary Practice: Case Report
Elena Yu Zakirova, Dmitry V. Shalimov , Ekaterina E. Garanina, Margarita N. Zhuravleva, Catrin S. Rutland and Albert A. Rizvanov, Frontiers in Veterinary Science, v. 6, article 76, March 2019.


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