Elena Yu Zakirova
Use of biologically active 3D matrix for extensive skin defect treatment in veterinary practice: case report
Zakirova, Elena Yu; Shalimov, Dmitry V.; Garanina, Ekaterina E.; Zhuravleva, Margarita N.; Rutland, Catrin S.; Rizvanov, Albert A.
Authors
Dmitry V. Shalimov
Ekaterina E. Garanina
Margarita N. Zhuravleva
CATRIN RUTLAND CATRIN.RUTLAND@NOTTINGHAM.AC.UK
Associate Professor
Albert A. Rizvanov
Abstract
Objectives: Large full-thickness skin defects represent a serious veterinary problem.
Methods: We have developed novel bioactive 3D-matrixes based on fibrin glue Tissucol (Baxter), containing the combination of the adenoviral constructs with genes vascular endothelial growth factor 165 (VEGF165) and fibroblast growth factor two (FGF2; construct Ad5-VEGF165 + Ad5-FGF2) or multipotent mesenchymal stem cells, genetically modified with these constructs.
Results: In vitro studies confirmed the biosynthesis of VEGF165 and FGF2 mRNA in the transduced cells. Ad5-VEGF165 + Ad5-FGF2- transduced multipotent mesenchymal stem cells showed an enhanced capacity to form capillary-like tubes in vitro. Bioactive 3D-matrix application enhanced granulation tissue formation and epithelialization of non-healing, large bite wounds in a dog. Successful wound healing was observed with a positive clinical outcome for the canine patient. This research and application of regenerative gene therapy alongside a novel bioactive 3D-matrix shows promising clinical applications for the future in both dogs and other mammals including humans.
Citation
Zakirova, E. Y., Shalimov, D. V., Garanina, E. E., Zhuravleva, M. N., Rutland, C. S., & Rizvanov, A. A. (2019). Use of biologically active 3D matrix for extensive skin defect treatment in veterinary practice: case report. Frontiers in Veterinary Science, 6, Article 76. https://doi.org/10.3389/fvets.2019.00076
Journal Article Type | Article |
---|---|
Acceptance Date | Feb 21, 2019 |
Online Publication Date | Mar 15, 2019 |
Publication Date | Mar 15, 2019 |
Deposit Date | Mar 18, 2019 |
Publicly Available Date | Mar 18, 2019 |
Journal | Frontiers in Veterinary Science |
Print ISSN | 2297-1769 |
Electronic ISSN | 2297-1769 |
Publisher | Frontiers Media |
Peer Reviewed | Peer Reviewed |
Volume | 6 |
Article Number | 76 |
DOI | https://doi.org/10.3389/fvets.2019.00076 |
Public URL | https://nottingham-repository.worktribe.com/output/1661981 |
Publisher URL | https://www.frontiersin.org/articles/10.3389/fvets.2019.00076/full |
Additional Information | Use of Biologically Active 3D Matrix for Extensive Skin Defect Treatment in Veterinary Practice: Case Report Elena Yu Zakirova, Dmitry V. Shalimov , Ekaterina E. Garanina, Margarita N. Zhuravleva, Catrin S. Rutland and Albert A. Rizvanov, Frontiers in Veterinary Science, v. 6, article 76, March 2019. |
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Use of biologically active 3D matrix for extensive skin defect treatment in veterinary practice: case report
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Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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