Function-related replacement of bacterial siderophore pathways

Moore, Bradley S; Jensen, Paul R; Letzel, Anne-Catrin; Cr�semann, Max; Bruns, Hilke; Crusemann, Max; Letzel, Anne Catrin; Alanjary, Mohammad; McInerney, James O; Jensen, Paul R.; Schulz, Stefan; Moore, Bradley S.; Ziemert, Nadine

Abstract

© The Author(s) 2018. Bacterial genomes are rife with orphan biosynthetic gene clusters (BGCs) associated with secondary metabolism of unrealized natural product molecules. Often up to a tenth of the genome is predicted to code for the biosynthesis of diverse metabolites with mostly unknown structures and functions. This phenomenal diversity of BGCs coupled with their high rates of horizontal transfer raise questions about whether they are really active and beneficial, whether they are neutral and confer no advantage, or whether they are carried in genomes because they are parasitic or addictive. We previously reported that Salinispora bacteria broadly use the desferrioxamine family of siderophores for iron acquisition. Herein we describe a new and unrelated group of peptidic siderophores called salinichelins from a restricted number of Salinispora strains in which the desferrioxamine biosynthesis genes have been lost. We have reconstructed the evolutionary history of these two different siderophore families and show that the acquisition and retention of the new salinichelin siderophores co-occurs with the loss of the more ancient desferrioxamine pathway. This identical event occurred at least three times independently during the evolution of the genus. We surmise that certain BGCs may be extraneous because of their functional redundancy and demonstrate that the relative evolutionary pace of natural pathway replacement shows high selective pressure against retention of functionally superfluous gene clusters.

Citation

Moore, B. S., Jensen, P. R., Letzel, A.-C., Crüsemann, M., Bruns, H., Crusemann, M., Letzel, A. C., Alanjary, M., McInerney, J. O., Jensen, P. R., Schulz, S., Moore, B. S., & Ziemert, N. (2018). Function-related replacement of bacterial siderophore pathways. ISME Journal, 12(2), 320-329. https://doi.org/10.1038/ismej.2017.137

• Journal Article Type: Article
• Acceptance Date: Jul 14, 2017
• Online Publication Date: Aug 15, 2017
• Publication Date: 2018-02
• Deposit Date: Nov 12, 2019
• Publicly Available Date: Jan 20, 2020
• Journal: The ISME Journal
• Print ISSN: 1751-7362
• Electronic ISSN: 1751-7370
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 12
• Issue: 2
• Pages: 320-329
• DOI: https://doi.org/10.1038/ismej.2017.137
• Public URL: https://nottingham-repository.worktribe.com/output/1637729
• Publisher URL: https://www.nature.com/articles/ismej2017137
• Related Public URLs: http://www.ncbi.nlm.nih.gov/pubmed/28809850
• Additional Information: Received: 10 March 2017; Revised: 13 June 2017; Accepted: 14 July 2017; First Online: 15 August 2017; : The authors declare no conflict of interest.
• Contract Date: Nov 12, 2019