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Biosynthetic bifunctional enzyme complex with high-efficiency luciferin-recycling to enhance the bioluminescence imaging

Hill, Philip; Sun, Xiaohui; Tang, Xu; Hu, Rui; Luo, Man; HILL, PHIL; Fang, Baishan; Xu, Chang'an

Biosynthetic bifunctional enzyme complex with high-efficiency luciferin-recycling to enhance the bioluminescence imaging Thumbnail


Authors

Philip Hill

Xiaohui Sun

Xu Tang

Rui Hu

Man Luo

PHIL HILL phil.hill@nottingham.ac.uk
Associate Professor

Baishan Fang

Chang'an Xu



Abstract

Firefly luciferase is a prominent reporter on molecular imaging with the advantage of longer wavelength on light emission and the ATP linear correlation, which makes it useful in most of current bioluminescence imaging model. However, the utility of this biomaterial was limited by the signal intensity and stability which are respectively affected by enzyme activity and substrate consumption. This study demonstrated a series of novel synthetic bifunctional enzyme complex of Firefly luciferase (Fluc) and Luciferin-regenerating enzyme (LRE). A peptide linker library was constructed for the fusion strategy on biosynthesis. The findings of both experimental data and structural simulation demonstrated that the intervention of fused LRE remarkably improve the stability of in vitro bioluminescence signal through luciferin recycling; and revealed the competitive relationship of Fluc and LRE on luciferin binding: Fluc performed higher activity with one copy number of rigid linker (EAAAK) at the C terminal while LRE acted more efficiently with two copy numbers of flexible linker (GGGGS) at the N terminal. With the advantage of signal intensity and stability, this fused bifunctional enzyme complex may expand the application of firefly luciferase to in vitro bioluminescence imaging.

Citation

Hill, P., Sun, X., Tang, X., Hu, R., Luo, M., HILL, P., …Xu, C. (2019). Biosynthetic bifunctional enzyme complex with high-efficiency luciferin-recycling to enhance the bioluminescence imaging. International Journal of Biological Macromolecules, 130, 705-714. https://doi.org/10.1016/j.ijbiomac.2019.03.036

Journal Article Type Article
Acceptance Date Mar 4, 2019
Online Publication Date Mar 5, 2019
Publication Date Jun 1, 2019
Deposit Date Mar 11, 2019
Publicly Available Date Mar 29, 2024
Journal International Journal of Biological Macromolecules
Print ISSN 0141-8130
Electronic ISSN 1879-0003
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 130
Pages 705-714
DOI https://doi.org/10.1016/j.ijbiomac.2019.03.036
Keywords Biochemistry; Molecular Biology; Structural Biology; General Medicine
Public URL https://nottingham-repository.worktribe.com/output/1624028
Publisher URL https://www.sciencedirect.com/science/article/pii/S0141813019303976?via%3Dihub
Additional Information This article is maintained by: Elsevier; Article Title: Biosynthetic bifunctional enzyme complex with high-efficiency luciferin-recycling to enhance the bioluminescence imaging; Journal Title: International Journal of Biological Macromolecules; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.ijbiomac.2019.03.036; Content Type: article; Copyright: © 2019 Elsevier B.V. All rights reserved.

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