Skip to main content

Research Repository

Advanced Search

Independent and combined effects of diethylhexyl phthalate and polychlorinated biphenyl 153 on sperm quality in the human and dog

Sumner, Rebecca N.; Tomlinson, Mathew; Craigon, Jim; England, Gary C. W.; Lea, Richard G.

Authors

Rebecca N. Sumner

Mathew Tomlinson

Jim Craigon

GARY ENGLAND gary.england@nottingham.ac.uk
Foundation Dean & Prof Comparative Veterinary Reproduction

RICHARD LEA richard.lea@nottingham.ac.uk
Associate Professor and Reader Inreproductive Biology



Abstract

A temporal decline in human and dog sperm quality is thought to reflect a common environmental aetiology. This may reflect direct effects of seminal chemicals on sperm function and quality. Here we report the effects of diethylhexyl phthalate (DEHP) and polychlorinated biphenyl 153 (PCB153) on DNA fragmentation and motility in human and dog sperm. Human and dog semen was collected from registered donors (n = 9) and from stud dogs (n = 11) and incubated with PCB153 and DEHP, independently and combined, at 0x, 2x, 10x and 100x dog testis concentrations. A total of 16 treatments reflected a 4 × 4 factorial experimental design. Although exposure to DEHP and/or PCB153 alone increased DNA fragmentation and decreased motility, the scale of dose-related effects varied with the presence and relative concentrations of each chemical (DEHP.PCB interaction for: DNA fragmentation; human p < 0.001, dog p < 0.001; Motility; human p < 0.001, dog p < 0.05). In both human and dog sperm, progressive motility negatively correlated with DNA fragmentation regardless of chemical presence (Human: P < 0.0001, r = −0.36; dog P < 0.0001, r = −0.29). We conclude that DEHP and PCB153, at known tissue concentrations, induce similar effects on human and dog sperm supporting the contention of the dog as a sentinel species for human exposure.

Journal Article Type Article
Publication Date Mar 4, 2019
Journal Scientific Reports
Print ISSN 2045-2322
Electronic ISSN 2045-2322
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 9
Article Number 3409
APA6 Citation Sumner, R. N., Tomlinson, M., Craigon, J., England, G. C. W., & Lea, R. G. (2019). Independent and combined effects of diethylhexyl phthalate and polychlorinated biphenyl 153 on sperm quality in the human and dog. Scientific Reports, 9, https://doi.org/10.1038/s41598-019-39913-9
DOI https://doi.org/10.1038/s41598-019-39913-9
Keywords Multidisciplinary
Publisher URL https://www.nature.com/articles/s41598-019-39913-9

Files

Independent and combined effects of diethylhexyl phthalate and polychlorinated biphenyl 153 on sperm quality in the human and dog (2.2 Mb)
PDF



You might also like



Downloadable Citations

;