Independent and combined effects of diethylhexyl phthalate and polychlorinated biphenyl 153 on sperm quality in the human and dog

Sumner, Rebecca N.; Tomlinson, Mathew; Craigon, Jim; England, Gary C. W.; Lea, Richard G.

Abstract

A temporal decline in human and dog sperm quality is thought to reflect a common environmental aetiology. This may reflect direct effects of seminal chemicals on sperm function and quality. Here we report the effects of diethylhexyl phthalate (DEHP) and polychlorinated biphenyl 153 (PCB153) on DNA fragmentation and motility in human and dog sperm. Human and dog semen was collected from registered donors (n = 9) and from stud dogs (n = 11) and incubated with PCB153 and DEHP, independently and combined, at 0x, 2x, 10x and 100x dog testis concentrations. A total of 16 treatments reflected a 4 × 4 factorial experimental design. Although exposure to DEHP and/or PCB153 alone increased DNA fragmentation and decreased motility, the scale of dose-related effects varied with the presence and relative concentrations of each chemical (DEHP.PCB interaction for: DNA fragmentation; human p < 0.001, dog p < 0.001; Motility; human p < 0.001, dog p < 0.05). In both human and dog sperm, progressive motility negatively correlated with DNA fragmentation regardless of chemical presence (Human: P < 0.0001, r = −0.36; dog P < 0.0001, r = −0.29). We conclude that DEHP and PCB153, at known tissue concentrations, induce similar effects on human and dog sperm supporting the contention of the dog as a sentinel species for human exposure.

Citation

Sumner, R. N., Tomlinson, M., Craigon, J., England, G. C. W., & Lea, R. G. (2019). Independent and combined effects of diethylhexyl phthalate and polychlorinated biphenyl 153 on sperm quality in the human and dog. Scientific Reports, 9, Article 3409. https://doi.org/10.1038/s41598-019-39913-9

• Journal Article Type: Article
• Acceptance Date: Feb 4, 2019
• Online Publication Date: Mar 4, 2019
• Publication Date: Mar 4, 2019
• Deposit Date: Mar 6, 2019
• Publicly Available Date: Mar 6, 2019
• Journal: Scientific Reports
• Electronic ISSN: 2045-2322
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 9
• Article Number: 3409
• DOI: https://doi.org/10.1038/s41598-019-39913-9
• Keywords: Multidisciplinary
• Public URL: https://nottingham-repository.worktribe.com/output/1612885
• Publisher URL: https://www.nature.com/articles/s41598-019-39913-9
• Contract Date: Mar 6, 2019