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Independent and combined effects of diethylhexyl phthalate and polychlorinated biphenyl 153 on sperm quality in the human and dog

Sumner, Rebecca N.; Tomlinson, Mathew; Craigon, Jim; England, Gary C. W.; Lea, Richard G.

Independent and combined effects of diethylhexyl phthalate and polychlorinated biphenyl 153 on sperm quality in the human and dog Thumbnail


Authors

Mathew Tomlinson

Jim Craigon

GARY ENGLAND gary.england@nottingham.ac.uk
Foundation Dean & Prof Comparative Veterinary Reproduction

RICHARD LEA richard.lea@nottingham.ac.uk
Professor of Reproductive Biology



Abstract

A temporal decline in human and dog sperm quality is thought to reflect a common environmental aetiology. This may reflect direct effects of seminal chemicals on sperm function and quality. Here we report the effects of diethylhexyl phthalate (DEHP) and polychlorinated biphenyl 153 (PCB153) on DNA fragmentation and motility in human and dog sperm. Human and dog semen was collected from registered donors (n = 9) and from stud dogs (n = 11) and incubated with PCB153 and DEHP, independently and combined, at 0x, 2x, 10x and 100x dog testis concentrations. A total of 16 treatments reflected a 4 × 4 factorial experimental design. Although exposure to DEHP and/or PCB153 alone increased DNA fragmentation and decreased motility, the scale of dose-related effects varied with the presence and relative concentrations of each chemical (DEHP.PCB interaction for: DNA fragmentation; human p < 0.001, dog p < 0.001; Motility; human p < 0.001, dog p < 0.05). In both human and dog sperm, progressive motility negatively correlated with DNA fragmentation regardless of chemical presence (Human: P < 0.0001, r = −0.36; dog P < 0.0001, r = −0.29). We conclude that DEHP and PCB153, at known tissue concentrations, induce similar effects on human and dog sperm supporting the contention of the dog as a sentinel species for human exposure.

Citation

Sumner, R. N., Tomlinson, M., Craigon, J., England, G. C. W., & Lea, R. G. (2019). Independent and combined effects of diethylhexyl phthalate and polychlorinated biphenyl 153 on sperm quality in the human and dog. Scientific Reports, 9, Article 3409. https://doi.org/10.1038/s41598-019-39913-9

Journal Article Type Article
Acceptance Date Feb 4, 2019
Online Publication Date Mar 4, 2019
Publication Date Mar 4, 2019
Deposit Date Mar 6, 2019
Publicly Available Date Mar 6, 2019
Journal Scientific Reports
Electronic ISSN 2045-2322
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 9
Article Number 3409
DOI https://doi.org/10.1038/s41598-019-39913-9
Keywords Multidisciplinary
Public URL https://nottingham-repository.worktribe.com/output/1612885
Publisher URL https://www.nature.com/articles/s41598-019-39913-9

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