Developing a high-throughput SNP-based marker system to facilitate the introgression of traits from Aegilops species into bread wheat (Triticum aestivum)

Przewieslik-Allen, Alexandra M.; Burridge, Amanda J.; Wilkinson, Paul A.; Winfield, Mark O.; Shaw, Daniel S.; McAusland, Lorna; King, Julie; King, Ian P.; Edwards, Keith J.; Barker, Gary L.A.

doi:10.3389/fpls.2018.01993

Developing a high-throughput SNP-based marker system to facilitate the introgression of traits from Aegilops species into bread wheat (Triticum aestivum)

Przewieslik-Allen, Alexandra M.; Burridge, Amanda J.; Wilkinson, Paul A.; Winfield, Mark O.; Shaw, Daniel S.; McAusland, Lorna; King, Julie; King, Ian P.; Edwards, Keith J.; Barker, Gary L.A.

Authors

Alexandra M. Przewieslik-Allen

Amanda J. Burridge

Paul A. Wilkinson

Mark O. Winfield

Daniel S. Shaw

Dr LORNA MCAUSLAND LORNA.MCAUSLAND@NOTTINGHAM.AC.UK
RESEARCH FELLOW

Professor JULIE KING julie.king@nottingham.ac.uk
PROFESSOR OF CEREAL GENETICS

Ian P. King

Keith J. Edwards

Gary L.A. Barker

Abstract

The genus Aegilops contains a diverse collection of wild species exhibiting variation in geographical distribution, ecological adaptation, ploidy and genome organization. Aegilops is the most closely related genus to Triticum which includes cultivated wheat, a globally important crop that has a limited gene pool for modern breeding. Aegilops species are a potential future resource for wheat breeding for traits, such as adaptation to different ecological conditions and pest and disease resistance. This study describes the development and application of the first high-throughput genotyping platform specifically designed for screening wheat relative species. The platform was used to screen multiple accessions representing all species in the genus Aegilops. Firstly, the data was demonstrated to be useful for screening diversity and examining relationships within and between Aegilops species. Secondly, markers able to characterize and track introgressions from Aegilops species in hexaploid wheat were identified and validated using two different approaches.

Citation

Przewieslik-Allen, A. M., Burridge, A. J., Wilkinson, P. A., Winfield, M. O., Shaw, D. S., McAusland, L., King, J., King, I. P., Edwards, K. J., & Barker, G. L. (2019). Developing a high-throughput SNP-based marker system to facilitate the introgression of traits from Aegilops species into bread wheat (Triticum aestivum). Frontiers in Plant Science, 9, Article 1993. https://doi.org/10.3389/fpls.2018.01993

Journal Article Type	Article
Acceptance Date	Dec 21, 2018
Online Publication Date	Jan 24, 2019
Publication Date	Jan 24, 2019
Deposit Date	Feb 28, 2019
Publicly Available Date	Mar 1, 2019
Journal	Frontiers in Plant Science
Electronic ISSN	1664-462X
Publisher	Frontiers Media
Peer Reviewed	Peer Reviewed
Volume	9
Article Number	1993
DOI	https://doi.org/10.3389/fpls.2018.01993
Keywords	Aegilops; Wheat, genotyping array; Single nucleotide polymorphism (SNP); Introgression; Wheat relative
Public URL	https://nottingham-repository.worktribe.com/output/1593072
Publisher URL	https://www.frontiersin.org/articles/10.3389/fpls.2018.01993/full
Contract Date	Mar 1, 2019