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Identification of Bio-oil Compound Utilizing Yeasts Through Phenotypic Microarray Screening

Kostas, Emily T.; Cooper, Mick; Shepherd, Benjamin J.; Robinson, John P.


Emily T. Kostas

Mick Cooper

Benjamin J. Shepherd

John P. Robinson


© 2019, The Author(s). Abstract: Biomass pyrolysis bio-oil contains a plethora of carbon sources with the potential to be utilized by microorganisms and converted into high value products. However, the majority of these compounds are either highly toxic to microorganisms or are not directly utilizable. Hence research is required to develop methods of separating microbe friendly compounds from inhibitory ones, and to also identify novel microorganisms that can utilise the principal carbon sources in pyrolysis bio-oil. This study employed a phenotypic microarray (PM) technique that measured yeast metabolic output to screen for and shortlist yeast strains able to metabolize various bio-oil carbon sources, with a focus on the anhydrosugar levoglucosan. Four strains of yeast (two Pichia spp. and two Kluyveromyces spp.) were shortlisted due to their high metabolic output (between 79.7 and 113.7 redox signal intensity) on levoglucosan from the PM assay. Under anaerobic fermentation conditions the strains were able to uptake levoglucosan (between 79 and 100% uptake efficiency) but not produce bioethanol; yet trace amounts of acetic acid were generated. This study demonstrated the application of applying the PM technique to screen for novel yeast strains with abilities to metabolize compounds present in pyrolysis bio-oil that could lead to the identification of novel levoglucosan utilization pathways. Graphical Abstract: [Figure not available: see fulltext.].


Kostas, E. T., Cooper, M., Shepherd, B. J., & Robinson, J. P. (2020). Identification of Bio-oil Compound Utilizing Yeasts Through Phenotypic Microarray Screening. Waste and Biomass Valorization, 11, 2507-2519.

Journal Article Type Article
Acceptance Date Feb 21, 2019
Online Publication Date Feb 26, 2019
Publication Date 2020-06
Deposit Date Feb 27, 2019
Publicly Available Date Feb 27, 2019
Journal Waste and Biomass Valorization
Print ISSN 1877-2641
Electronic ISSN 1877-265X
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 11
Pages 2507-2519
Keywords Phenotypic microarray; Yeast; Pyrolysis; Bio-oil compound; Fermentation
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