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Development of stable homozygous wheat/Amblyopyrum muticum (Aegilops mutica) introgression lines and their cytogenetic and molecular characterization

King, Julie; Newell, Claire; Grewal, Surbhi; Hubbart-Edwards, Stella; Yang, Cai-yun; Scholefield, Duncan; Ashling, Stephen; Stride, Alex; King, Ian P.

Authors

Julie King

Claire Newell

Surbhi Grewal

Stella Hubbart-Edwards

Cai-yun Yang

Duncan Scholefield

Stephen Ashling

Alex Stride

Ian P. King



Abstract

Wheat is one of the world’s most important sources of food. However, due to its evolution its genetic base has narrowed, which is severely limiting the ability of breeders to develop new higher yielding varieties that can adapt to the changing environment. In contrast to wheat, its wild relatives provide a vast reservoir of genetic variability for most, if not all, agronomically important traits. Genetic variation has previously been transferred to wheat from one of its wild relatives, Ambylopyrum muticum (previously known as Aegilops mutica). However, before the genetic variation available in this species can be assessed and exploited in breeding and for research, the transmission of the chromosome segments introgressed into wheat must first be stabilized. In this paper we describe the generation of 66 stably inherited homozygous wheat/Am. muticum introgression lines using a doubled haploid procedure. The characterisation and stability of each of these lines was determined via genomic in situ hybridization and SNP analysis. While most of the doubled haploid lines were found to carry only single introgressions, six lines carried two. Three lines carried only complete Am. muticum chromosomes, 43 carried only small or very small introgressions and the remainder carried either only large introgressions or a large plus a small introgression. The strategy that we are employing for the distribution and exploitation of the genetic variation from Am. muticum and a range of other species is discussed.

Journal Article Type Article
Publication Date Jan 31, 2019
Journal Frontiers in Plant Science
Electronic ISSN 1664-462X
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 10
Article Number 34
Pages 1-10
APA6 Citation King, J., Newell, C., Grewal, S., Hubbart-Edwards, S., Yang, C., Scholefield, D., …King, I. P. (2019). Development of stable homozygous wheat/Amblyopyrum muticum (Aegilops mutica) introgression lines and their cytogenetic and molecular characterization. Frontiers in Plant Science, 10, 1-10. doi:10.3389/fpls.2019.00034
DOI https://doi.org/10.3389/fpls.2019.00034
Keywords Plant Science
Publisher URL https://www.frontiersin.org/articles/10.3389/fpls.2019.00034/full

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