The effect of Amine modification on siRNA delivery of redox-responsive PEGylated amphiphilic micellar nanoparticles for triple negative breast cancer therapy

Mehradnia, Fatemeh; Alexander, Cameron

Abstract

We developed a set of methoxypoly(ethyleneglycol)-co-poly(N3-ε-caprolactone) (mPEG-N3PCL)copolymers with different types of amine linkers and investigated thecontribution of the introduced amino linker to the gene delivery efficiency ofnanoparticles. The nanoparticles were crosslinked from the caprolactone regionswith a redox-responsive linker. Formulation variables including Redox-crosslinkingand N:P ratio were examined to obtain nanoparticles with optimal size andhighest siRNA entrapment efficacy (EE). The nanoparticles were characterized byDLS, and nanodrop UV-vis spectroscopy.

Nanoparticle size, polydispersity index and siRNAentrapment efficacy were found to depend on the all the examined formulationvariables specially the N:P ratio. The formulation with N:P ratio of 5 with56.13 nm in size and 92.24% EE was chosen as the optimal formulation.

Controllable size, loading efficiency and releasepattern by redox-responsivity make this class of novel carriers a promisingcandidate for tumour targeted gene delivery applications.

Citation

Mehradnia, F., & Alexander, C. (2022). The effect of Amine modification on siRNA delivery of redox-responsive PEGylated amphiphilic micellar nanoparticles for triple negative breast cancer therapy. British Journal of Pharmacy, 7(2), https://doi.org/10.5920/bjpharm.1119

• Journal Article Type: Article
• Acceptance Date: Jul 4, 2022
• Online Publication Date: Nov 3, 2022
• Publication Date: Nov 3, 2022
• Deposit Date: Dec 14, 2022
• Publicly Available Date: Dec 14, 2022
• Journal: 2022 APS Special Issue
• Electronic ISSN: 2058-8356
• Publisher: University of Huddersfield Press
• Peer Reviewed: Peer Reviewed
• Volume: 7
• Issue: 2
• DOI: https://doi.org/10.5920/bjpharm.1119
• Keywords: General Medicine
• Public URL: https://nottingham-repository.worktribe.com/output/14888054
• Publisher URL: https://www.bjpharm.org.uk/article/id/1119/
• Additional Information: Proceedings of the 13th APS International PharmSci 2022