Label-free, high resolution, multi-modal light microscopy for discrimination of live stem cell differentiation status

Zhang, Jing; Moradi, Emilia; Somekh, Michael G.; Mather, Melissa L.

Abstract

A label-free microscopy method for assessing the differentiation status of stem cells is presented with potential application for characterization of therapeutic stem cell populations. The microscopy system is capable of characterizing live cells based on the use of evanescent wave microscopy and quantitative phase contrast (QPC) microscopy. The capability of the microscopy system is demonstrated by studying the differentiation of live immortalised neonatal mouse neural stem cells over a 15 day time course. Metrics extracted from microscope images are assessed and images compared with results from endpoint immuno-staining studies to illustrate the system’s performance. Results demonstrate the potential of the microscopy system as a valuable tool for cell biologists to readily identify the differentiation status of unlabelled live cells.

Citation

Zhang, J., Moradi, E., Somekh, M. G., & Mather, M. L. (2018). Label-free, high resolution, multi-modal light microscopy for discrimination of live stem cell differentiation status. Scientific Reports, 8(1), Article 697. https://doi.org/10.1038/s41598-017-18714-y

• Journal Article Type: Article
• Acceptance Date: Dec 15, 2017
• Online Publication Date: Jan 15, 2018
• Publication Date: Dec 1, 2018
• Deposit Date: Jan 10, 2019
• Publicly Available Date: Jan 11, 2019
• Journal: Scientific Reports
• Print ISSN: 2045-2322
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 8
• Issue: 1
• Article Number: 697
• DOI: https://doi.org/10.1038/s41598-017-18714-y
• Public URL: https://nottingham-repository.worktribe.com/output/1463182
• Publisher URL: https://www.nature.com/articles/s41598-017-18714-y