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Cloning, Expression, and Functional Analysis of Patient-Derived Hepatitis C Virus Glycoproteins

Tarr, Alexander W.; Owsianka, Ania M.; Szwejk, Alexandra; Ball, Jonathan K.; Patel, Arvind H.

Authors

Ania M. Owsianka

Alexandra Szwejk

JONATHAN BALL jonathan.ball@nottingham.ac.uk
Professor of Molecular Virology

Arvind H. Patel



Abstract

Hepatitis C virus (HCV) infection is a major cause of severe chronic liver disease including cirrhosis and hepatocellular carcinoma. HCV has been classified into six major genotypes that exhibit extensive genetic variability, particularly in the envelope glycoproteins El and E2. Knowledge of genotypic and quasispecies variation on viral glycoprotein properties is important in understanding the structure-function relationship of the proteins. Through their perceived role as components of the virion and mediators of virus attachment and entry, HCV glycoproteins are primary targets for the development of antiviral agents. In this chapter, we describe methods optimized to extract E1E2-encoding sequences of all the major genotypes from HCV-infected patient sera, and their amplification, cloning, expression, and biochemical characterization. Furthermore, we describe a method to generate retroviral nucleocapsid pseudotyped with HCV E1E2 of diverse genotypes (HCVpp) whereby infectivity of the retroviral particle is conferred by HCV glycoproteins. Finally, we show how the HCVpp can be used in an infection assay to determine the viral glycoprotein function at the level of the host-pathogen interface and subsequent events leading to virus infection. © Humana Press Inc.

Publication Date Feb 2, 2007
Deposit Date Nov 25, 2022
Publisher Humana Press
Pages 177-197
Series Title Methods in Molecular Biology
Series Number 379
Book Title Glycovirology Protocols
ISBN 978-1-58829-590-3
DOI https://doi.org/10.1385/1-59745-393-5%3A177
Public URL https://nottingham-repository.worktribe.com/output/14040046
Publisher URL https://link.springer.com/protocol/10.1007/978-1-59745-393-6_13