A Spatiotemporal DNA Endoploidy Map of the Arabidopsis Root Reveals Roles for the Endocycle in Root Development and Stress Adaptation

Bhosale, Rahul; Boudolf, Veronique; Cuevas, Fabiola; Lu, Ran; Eekhout, Thomas; Hu, Zhubing; Van Isterdael, Gert; Lambert, Georgina M.; Xu, Fan; Nowack, Moritz K.; Smith, Richard S.; Vercauteren, Ilse; De Rycke, Riet; Storme, Veronique; Beeckman, Tom; Larkin, John C.; Kremer, Anna; Galbraith, David W.; Kumpf, Robert P.; Maere, Steven; De Veylder, Lieven

doi:10.1105/tpc.17.00983

A Spatiotemporal DNA Endoploidy Map of the Arabidopsis Root Reveals Roles for the Endocycle in Root Development and Stress Adaptation

Authors

RAHUL BHOSALE RAHUL.BHOSALE@NOTTINGHAM.AC.UK
Assistant Professor

Veronique Boudolf

Fabiola Cuevas

Ran Lu

Thomas Eekhout

Zhubing Hu

Gert Van Isterdael

Georgina M. Lambert

Fan Xu

Moritz K. Nowack

Richard S. Smith

Ilse Vercauteren

Riet De Rycke

Veronique Storme

Tom Beeckman

John C. Larkin

Anna Kremer

Herman

David W. Galbraith

Robert P. Kumpf

Steven Maere

Lieven De Veylder

Abstract

© 2018 ASPB. Somatic polyploidy caused by endoreplication is observed in arthropods, molluscs, and vertebrates but is especially prominent in higher plants, where it has been postulated to be essential for cell growth and fate maintenance. However, a comprehensive understanding of the physiological significance of plant endopolyploidy has remained elusive. Here, we modeled and experimentally verified a high-resolution DNA endoploidy map of the developing Arabidopsis thaliana root, revealing a remarkable spatiotemporal control of DNA endoploidy levels across tissues. Fitting of a simplified model to publicly available data sets profiling root gene expression under various environmental stress conditions suggested that this root endoploidy patterning may be stress-responsive. Furthermore, cellular and transcriptomic analyses revealed that inhibition of endoreplication onset alters the nuclear-to-cellular volume ratio and the expression of cell wall-modifying genes, in correlation with the appearance of cell structural changes. Our data indicate that endopolyploidy might serve to coordinate cell expansion with structural stability and that spatiotemporal endoreplication pattern changes may buffer for stress conditions, which may explain the widespread occurrence of the endocycle in plant species growing in extreme or variable environments.

Citation

Bhosale, R., Boudolf, V., Cuevas, F., Lu, R., Eekhout, T., Hu, Z., …De Veylder, L. (2018). A Spatiotemporal DNA Endoploidy Map of the Arabidopsis Root Reveals Roles for the Endocycle in Root Development and Stress Adaptation. Plant Cell, 30(10), 2330-2351. https://doi.org/10.1105/tpc.17.00983

Journal Article Type	Article
Acceptance Date	Aug 8, 2018
Online Publication Date	Aug 16, 2018
Publication Date	2018-10
Deposit Date	Dec 7, 2018
Journal	Plant Cell
Print ISSN	1040-4651
Electronic ISSN	1532-298X
Publisher	American Society of Plant Biologists
Peer Reviewed	Peer Reviewed
Volume	30
Issue	10
Pages	2330-2351
DOI	https://doi.org/10.1105/tpc.17.00983
Keywords	Plant Science; Cell Biology
Public URL	https://nottingham-repository.worktribe.com/output/1388612
Publisher URL	http://www.plantcell.org/content/30/10/2330