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A Spatiotemporal DNA Endoploidy Map of the Arabidopsis Root Reveals Roles for the Endocycle in Root Development and Stress Adaptation

Bhosale, Rahul; Boudolf, Veronique; Cuevas, Fabiola; Lu, Ran; Eekhout, Thomas; Hu, Zhubing; Van Isterdael, Gert; Lambert, Georgina M.; Xu, Fan; Nowack, Moritz K.; Smith, Richard S.; Vercauteren, Ilse; De Rycke, Riet; Storme, Veronique; Beeckman, Tom; Larkin, John C.; Kremer, Anna; Höfte, Herman; Galbraith, David W.; Kumpf, Robert P.; Maere, Steven; De Veylder, Lieven

Authors

Veronique Boudolf

Fabiola Cuevas

Ran Lu

Thomas Eekhout

Zhubing Hu

Gert Van Isterdael

Georgina M. Lambert

Fan Xu

Moritz K. Nowack

Richard S. Smith

Ilse Vercauteren

Riet De Rycke

Veronique Storme

Tom Beeckman

John C. Larkin

Anna Kremer

Herman Höfte

David W. Galbraith

Robert P. Kumpf

Steven Maere

Lieven De Veylder



Abstract

© 2018 ASPB. Somatic polyploidy caused by endoreplication is observed in arthropods, molluscs, and vertebrates but is especially prominent in higher plants, where it has been postulated to be essential for cell growth and fate maintenance. However, a comprehensive understanding of the physiological significance of plant endopolyploidy has remained elusive. Here, we modeled and experimentally verified a high-resolution DNA endoploidy map of the developing Arabidopsis thaliana root, revealing a remarkable spatiotemporal control of DNA endoploidy levels across tissues. Fitting of a simplified model to publicly available data sets profiling root gene expression under various environmental stress conditions suggested that this root endoploidy patterning may be stress-responsive. Furthermore, cellular and transcriptomic analyses revealed that inhibition of endoreplication onset alters the nuclear-to-cellular volume ratio and the expression of cell wall-modifying genes, in correlation with the appearance of cell structural changes. Our data indicate that endopolyploidy might serve to coordinate cell expansion with structural stability and that spatiotemporal endoreplication pattern changes may buffer for stress conditions, which may explain the widespread occurrence of the endocycle in plant species growing in extreme or variable environments.

Journal Article Type Article
Acceptance Date Aug 8, 2018
Online Publication Date Aug 16, 2018
Publication Date 2018-10
Deposit Date Dec 7, 2018
Journal The Plant Cell
Print ISSN 1040-4651
Electronic ISSN 1532-298X
Publisher American Society of Plant Biologists
Peer Reviewed Peer Reviewed
Volume 30
Issue 10
Pages 2330-2351
DOI https://doi.org/10.1105/tpc.17.00983
Keywords Plant Science; Cell Biology
Public URL https://nottingham-repository.worktribe.com/output/1388612
Publisher URL http://www.plantcell.org/content/30/10/2330