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Investigating the association between the caecal microbiomes of broilers and Campylobacter burden

Sakaridis, Ioannis; Ellis, Richard J.; Cawthraw, Shaun A.; van Vliet, Arnoud H. M.; Stekel, Dov J.; Penell, Johanna; Chambers, Mark; La Ragione, Roberto M.; Cook, Alasdair J.

Investigating the association between the caecal microbiomes of broilers and Campylobacter burden Thumbnail


Authors

Ioannis Sakaridis

Richard J. Ellis

Shaun A. Cawthraw

Arnoud H. M. van Vliet

DOV STEKEL DOV.STEKEL@NOTTINGHAM.AC.UK
Professor of Computational Biology

Johanna Penell

Mark Chambers

Roberto M. La Ragione

Alasdair J. Cook



Abstract

One of the major transmission routes for the foodborne bacterial pathogen Campylobacter is undercooked poultry meat, contaminated from intestinal contents during processing. In broilers, Campylobacter can grow to very high densities in the caeca, and is often considered to be a commensal or an opportunistic pathogen in poultry. Reduction of caecal loads of Campylobacter may assist in lowering incidence rates of Campylobacter food poisoning. To achieve this, there needs to be a better understanding of the dynamics of Campylobacter colonization in its natural niche, and the effect of the local microbiome on colonization. Previous studies have shown that the microbiome differed between Campylobacter colonized and non-colonized chicken intestinal samples. To characterize the microbiome of Campylobacter-colonized broilers, caecal samples of 100 randomly selected birds from four farms were analyzed using amplified 16S rRNA gene sequences. Bacterial taxonomic analysis indicated that inter-farm variation was greater than intra-farm variation. The two most common bacterial groups were Bacteroidetes and Firmicutes which were present in all samples and constituted 29.7–63.5 and 30.2–59.8% of the bacteria present, respectively. Campylobacter was cultured from all samples, ranging from 2 to 9 log10 CFU g-1. There was no clear link between Campylobacter counts and Firmicutes, Bacteroidetes, or Tenericutes levels in the 16S rRNA operational taxonomic unit (OTU)-based analysis of the caecal microbiome, but samples with high Campylobacter counts (>9 log CFU g-1) contained increased levels of Enterobacteriaceae. A decrease in Lactobacillus abundance in chicken caeca was also associated with high Campylobacter loads. The reported associations with Lactobacillus and Enterobacteriaceae match changes in the intestinal microbiome of chickens and mice previously reported for Campylobacter infection, and raises the question about temporality and causation; as to whether increases in Campylobacter loads create conditions adverse to Lactobacilli and/or beneficial to Enterobacteriaceae, or that changes in Lactobacilli and Enterobacteriaceae levels created conditions beneficial for Campylobacter colonization. If these changes can be controlled, this may open opportunities for modulation of chicken microbiota to reduce Campylobacter levels for improved food safety.

Journal Article Type Article
Acceptance Date Apr 20, 2018
Online Publication Date May 22, 2018
Publication Date May 22, 2018
Deposit Date Dec 3, 2018
Publicly Available Date Dec 4, 2018
Journal Frontiers in Microbiology
Electronic ISSN 1664-302X
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 9
Article Number 927
DOI https://doi.org/10.3389/fmicb.2018.00927
Public URL https://nottingham-repository.worktribe.com/output/1350015
Publisher URL https://www.frontiersin.org/articles/10.3389/fmicb.2018.00927/full

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