Genetically Encoded FRET Biosensors to Illuminate Compartmentalised GPCR Signalling

Halls, M.L.; Canals, M.

Abstract

Genetically encoded Förster resonance energy transfer (FRET) biosensors have been instrumental to our understanding of how intracellular signalling is organised and regulated within cells. In the last decade, the toolbox, dynamic range and applications of these sensors have expanded beyond basic cell biology applications. In particular, FRET biosensors have shed light onto the mechanisms that control the intracellular organisation of G protein-coupled receptor (GPCR) signalling and have allowed the visualisation of signalling events with unprecedented temporal and spatial resolution. Here we review the use of these sensors in the GPCR field and how it has already provided invaluable advances towards our understanding of the complexity of GPCR signalling. © 2017 Elsevier Ltd

Citation

Halls, M., & Canals, M. (2018). Genetically Encoded FRET Biosensors to Illuminate Compartmentalised GPCR Signalling. Trends in Pharmacological Sciences, 39(2), 148-157. https://doi.org/10.1016/j.tips.2017.09.005

• Journal Article Type: Article
• Acceptance Date: Oct 17, 2017
• Online Publication Date: Oct 17, 2017
• Publication Date: 2018-02
• Deposit Date: Jan 17, 2020
• Print ISSN: 0165-6147
• Electronic ISSN: 1873-3735
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 39
• Issue: 2
• Pages: 148-157
• DOI: https://doi.org/10.1016/j.tips.2017.09.005
• Keywords: compartmentalised signalling; Förster resonance energy transfer biosensors; G protein-coupled receptor; G protein coupled receptor; recombinant protein; animal; cell compartmentalization; chemistry; fluorescence resonance energy transfer; genetic procedures; genetics; human; metabolism; procedures; signal transduction; single molecule imaging; Animals; Biosensing Techniques; Cell Compartmentation; Fluorescence Resonance Energy Transfer; Humans; Receptors, G-Protein-Coupled; Recombinant Proteins; Signal Transduction; Single Molecule Imaging
• Public URL: https://nottingham-repository.worktribe.com/output/1311734
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0165614717301840
• Related Public URLs: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85031732930&doi=10.1016%2fj.tips.2017.09.005&partnerID=40&md5=69e2da863473bdef035d3956d067a5a2