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Genome-Wide Genetic Diversity and Population Structure of Local Sudanese Sheep Populations Revealed by Whole-Genome Sequencing

Amane, Agraw; Belay, Gurja; Tijjani, Abdulfatai; Dessie, Tadelle; Musa, Hassan H.; Hanotte, Olivier

Genome-Wide Genetic Diversity and Population Structure of Local Sudanese Sheep Populations Revealed by Whole-Genome Sequencing Thumbnail


Authors

Agraw Amane

Gurja Belay

Abdulfatai Tijjani

Tadelle Dessie

Hassan H. Musa

OLIVIER HANOTTE OLIVIER.HANOTTE@NOTTINGHAM.AC.UK
Director of Frozen Ark Project & Professor of Genetics & Conservation



Abstract

Local Sudanese sheep populations inhabiting diverse environmental conditions and holding opposing morphologies provide opportunities for molecular-genetic research. Characterizing their genome is crucial for sustainable breeding improvement and targeting favorable genes in breeding programs. However, the genome of these sheep populations, which comprises several subtypes, remains uncharacterized using whole-genome sequence data. This study aimed to elucidate genome-wide genetic diversity and population structure of 11 local Sudanese sheep populations, namely, Hammari, Kabbashi, Meidobe, Ashgar, Dubasi, Watish, Bega, Naili, Fulani, Zagawi, and Garag. Ninety whole blood samples were collected, and we extracted DNA using a Qiagen DNeasy® extraction kit. We used the Illumina HiSeq 2000 platform to sequence all the DNA samples. We included whole-genome sequence data of three Ethiopian sheep (Doyogena, Kefis, and Gafera) and one Libyan sheep (Libyan Barbary) in the study to infer the genetic relationships of local Sudanese sheep populations from a continental perspective. A total of 44.8 million bi-allelic autosomal SNPs were detected; 28.5% and 63.3% occur in introns and intergenic regions, respectively. The mean genetic diversity ranged from 0.276 for Garag to 0.324 for Kabbashi sheep populations. The lowest FST estimates were observed between Kabbashi and Ashgar and the highest between Bega and Fulani local Sudanese sheep populations. The principal component and population structure analyses of the 11 local Sudanese sheep populations indicated three separate genetic groups categorized following their tail morphotype, geographical distribution, and population subtype. The thin-tailed local Sudanese sheep populations exhibited independent clustering from the fat-tailed Ethiopian and Libyan sheep. We also observed distinct clustering between the fat-tailed Ethiopian and Libyan sheep. The present study’s findings demonstrated the population structure and principal components related to tail morphotype, geographical distribution, and population subtype of local Sudanese sheep populations. A clear signature of admixture was observed among the studied local Sudanese sheep populations.

Citation

Amane, A., Belay, G., Tijjani, A., Dessie, T., Musa, H. H., & Hanotte, O. (2022). Genome-Wide Genetic Diversity and Population Structure of Local Sudanese Sheep Populations Revealed by Whole-Genome Sequencing. Diversity, 14(11), Article 895. https://doi.org/10.3390/d14110895

Journal Article Type Article
Acceptance Date Oct 5, 2022
Online Publication Date Oct 22, 2022
Publication Date Oct 22, 2022
Deposit Date Dec 12, 2022
Publicly Available Date Dec 14, 2022
Journal Diversity
Electronic ISSN 1424-2818
Publisher MDPI AG
Peer Reviewed Peer Reviewed
Volume 14
Issue 11
Article Number 895
DOI https://doi.org/10.3390/d14110895
Keywords Nature and Landscape Conservation; Agricultural and Biological Sciences (miscellaneous); Ecological Modeling; Ecology
Public URL https://nottingham-repository.worktribe.com/output/12902231
Publisher URL https://www.mdpi.com/1424-2818/14/11/895

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