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Characterisation and comparison of temporal release profiles of nitric oxide generating donors

Bradley, Sophie A.; Steinert, Joern R.

Characterisation and comparison of temporal release profiles of nitric oxide generating donors Thumbnail


Authors

Sophie A. Bradley



Abstract

Background
Nitric oxide (NO) is a vital signalling molecule in a variety of tissues including the neuronal, vascular and reproductive system. However, its high diffusibility and inactivation make characterisation of nitrergic signalling difficult. The use of NO donors is essential to characterise downstream signalling pathways but knowledge of donor release capacities is lacking, thus making comparisons of donor responses difficult.

New method
This study characterises NO profiles of commonly used NO donors. Donors were stored under defined conditions and temporal release profiles detected to allow determination of released NO concentrations.

Results
Using NO-sensitive microsensors we assessed release profiles of NO donors following different storage times and conditions. We found that donors such as NOC-5 and PAPA-NONOate decayed substantially within days, whereas SNP and GSNO showed greater stability releasing consistent levels of NO over days. In all donors tested, the amount of released NO differs between frozen and unfrozen stocks.

Comparison with existing method(s)
Fluorescent and amperometric approaches to measure NO concentrations yield a wide range of levels. However, due to a lack of characterisation of the release profiles, inconsistent effects on NO signalling have been widely documented. Our systematic assessment of release profiles of a range of NO donors therefore provides new essential data allowing for improved and defined investigations of nitrergic signalling.

Conclusions
This is the first systematic comparison of temporal release profiles of different NO donors allowing researchers to compare conditions across different studies and the use of defined NO levels by choosing specific donors and concentrations.

Journal Article Type Article
Acceptance Date Feb 20, 2015
Online Publication Date Mar 6, 2023
Publication Date Apr 30, 2015
Deposit Date Jan 18, 2023
Publicly Available Date Jan 18, 2023
Journal Journal of Neuroscience Methods
Print ISSN 0165-0270
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 245
Pages 116-124
DOI https://doi.org/10.1016/j.jneumeth.2015.02.024
Keywords General Neuroscience
Public URL https://nottingham-repository.worktribe.com/output/12902118
Publisher URL https://www.sciencedirect.com/science/article/pii/S0165027015000825?via%3Dihub

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