David P. Richards
(−)-Adaline from the Adalia Genus of Ladybirds Is a Potent Antagonist of Insect and Specific Mammalian Nicotinic Acetylcholine Receptors
Richards, David P.; Patel, Rohit N.; Duce, Ian R.; Khambay, Bhupinder P. S.; Birkett, Michael A.; Pickett, John A.; Mellor, Ian R.
Authors
Rohit N. Patel
Ian R. Duce
Bhupinder P. S. Khambay
Michael A. Birkett
John A. Pickett
Dr IAN MELLOR IAN.MELLOR@NOTTINGHAM.AC.UK
ASSISTANT PROFESSOR
Abstract
Ladybird beetles (Coleoptera: Coccinellidae) possess strong chemical defences that are secreted in response to stress and are also found on the coating of eggs, which are rich in alkaloids that are responsible for their toxicity to other species. Recent studies have shown that alkaloids from several species of ladybird beetle can target nicotinic acetylcholine receptors (nAChRs) acting as receptor antagonists. Here, we have explored the actions of (−)-adaline, found in the 2-spot (Adalia bipunctata) and 10-spot (Adalia decempunctata) ladybirds, on both mammalian (α1β1γδ, α7, α4β2, α3β4) and insect nAChRs using patch-clamp of TE671 cells and locust brain neurons natively expressing nAChRs, as well as two-electrode voltage clamp of Xenopus laevis oocytes recombinantly expressing nAChRs. All nAChR subtypes were antagonised by (−)-adaline in a time-dependent, voltage-dependent and non-competitive manner with the lowest IC50s at rat α3β4 (0.10 μM) and locust neuron (1.28 μM) nAChRs, at a holding potential of −75 mV. The data imply that (−)-adaline acts as an open channel blocker of nAChRs.
Citation
Richards, D. P., Patel, R. N., Duce, I. R., Khambay, B. P. S., Birkett, M. A., Pickett, J. A., & Mellor, I. R. (2022). (−)-Adaline from the Adalia Genus of Ladybirds Is a Potent Antagonist of Insect and Specific Mammalian Nicotinic Acetylcholine Receptors. Molecules, 27(20), Article 7074. https://doi.org/10.3390/molecules27207074
Journal Article Type | Article |
---|---|
Acceptance Date | Oct 17, 2022 |
Online Publication Date | Oct 20, 2022 |
Publication Date | Oct 2, 2022 |
Deposit Date | Jan 11, 2023 |
Publicly Available Date | Jan 12, 2023 |
Journal | Molecules |
Electronic ISSN | 1420-3049 |
Publisher | MDPI |
Peer Reviewed | Peer Reviewed |
Volume | 27 |
Issue | 20 |
Article Number | 7074 |
DOI | https://doi.org/10.3390/molecules27207074 |
Keywords | Chemistry (miscellaneous); Analytical Chemistry; Organic Chemistry; Physical and Theoretical Chemistry; Molecular Medicine; Drug Discovery; Pharmaceutical Science |
Public URL | https://nottingham-repository.worktribe.com/output/12624562 |
Publisher URL | https://www.mdpi.com/1420-3049/27/20/7074 |
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(−)-Adaline from the Adalia Genus of Ladybirds Is a Potent Antagonist of Insect and Specific Mammalian Nicotinic Acetylcholine Receptors
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