Chlorophyll fluorescence on the fast timescale

Ajigboye, Olubukola O.; Ray, Rumiana V.; Murchie, Erik H.

Abstract

Chlorophyll fluorescence is a rapid and non-invasive tool used for probing the activity of photosynthesis that can be used in vivo and in the field. It is highly relevant to the demands of high-throughput crop phenotyping and can be automated or manually applied. Here we describe protocols and advice for making fast timescale fluorescence measurements using handheld equipment in the laboratory or in the field. While interpretation of some measured parameters requires caution, we demonstrate that this technique is appropriate for some applications where convenience, rapidity, and sensitivity are required.

Citation

Ajigboye, O. O., Ray, R. V., & Murchie, E. H. (2018). Chlorophyll fluorescence on the fast timescale. In S. Covshoff (Ed.), Photosynthesis: Methods and Protocols, 95-104. Springer Publishing Company. doi:10.1007/978-1-4939-7786-4_6

• Journal Article Type: Article
• Acceptance Date: Feb 22, 2018
• Online Publication Date: Jul 6, 2018
• Publication Date: Jul 6, 2018
• Deposit Date: Dec 6, 2018
• Publisher: Springer Publishing Company
• Peer Reviewed: Peer Reviewed
• Pages: 95-104
• Series Title: Methods in Molecular Biology
• Series Number: 1770
• Book Title: Photosynthesis: Methods and Protocols
• DOI: https://doi.org/10.1007/978-1-4939-7786-4_6
• Public URL: https://nottingham-repository.worktribe.com/output/1216607
• Publisher URL: https://link.springer.com/protocol/10.1007%2F978-1-4939-7786-4_6