Cytotoxic potential of ethanol extract of Parquetina nigrescens on MCF-7, C4-2WT, HT 29 and HTC 116 cell lines

M. Onyegeme-Okerenta, Blessing; Agyare, Christian; D. Bradshaw, Tracey; A. Spriggs, Keith

Abstract

The cytotoxic activity of ethanol extract of Parquetina nigrescen was investigated using a (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, methylene blue and Trypan Blue exclusion assay on four human cancer cell lines, MCF-7, HT 29, HTC 116 and C4-2WT. A mitochondrial enzyme in living cells, succinate-dehydrogenase cleaves the tetrazolium ring and converts the MTT to an insoluble purple formazan whose intensity is directly proportional to the presence of viable cells in the microwell plate. Results showed a significant (p less than 0.05) cytotoxic effect of the extract in a dose dependent manner. Cytotoxicity increased with increase in the concentration of the extract used. GI50 results calculated after MTT test showed the concentration of ethanol extract of P. nigrescens required for 50% inhibition of the different cell lines as follows: MCF-7 = 2.61 µg/ml, C4-2WT = 8.33 µg/ml, HCT 29 = 3.47 µg/ml and HCT 116 =1.75 µg/ml. For the methylene blue assay, the number of viable cells present was significantly reduced (p less than 0.05) with an increase in the concentration of the extract and duration of exposure of the cells to the extract. The result of trypan blue assay showed a significant reduction (p less than 0.05) in the total count of viable cells and a significant increase (p less than 0.05) in the total count of non-viable cells over 72 h post-treatment with an extract of P. nigrescens. Comparatively, results obtained indicate that there is a correlation between the various methods adopted in establishing the antiproliferative and cytotoxic activity of ethanol extract of P. nigrescens obtained in this study.

Citation

M. Onyegeme-Okerenta, B., Agyare, C., D. Bradshaw, T., & A. Spriggs, K. (2018). Cytotoxic potential of ethanol extract of Parquetina nigrescens on MCF-7, C4-2WT, HT 29 and HTC 116 cell lines. African journal of pharmacy and pharmacology, 12(23), 310-318. https://doi.org/10.5897/ajpp2018.4947

• Journal Article Type: Article
• Acceptance Date: Jul 16, 2018
• Online Publication Date: Aug 8, 2018
• Publication Date: Aug 8, 2018
• Deposit Date: Oct 29, 2018
• Publicly Available Date: Oct 29, 2018
• Journal: African Journal of Pharmacy and Pharmacology
• Print ISSN: 1996-0816
• Electronic ISSN: 1996-0816
• Peer Reviewed: Peer Reviewed
• Volume: 12
• Issue: 23
• Pages: 310-318
• DOI: https://doi.org/10.5897/ajpp2018.4947
• Keywords: Pharmacology; Pharmaceutical Science
• Public URL: https://nottingham-repository.worktribe.com/output/1204786
• Publisher URL: https://academicjournals.org/journal/AJPP/article-abstract/EAA2D9857998