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N6-methyladenosine inhibits local ribonucleolytic cleavage to stabilise mRNAs in Arabidopsis

Anderson, Stephen J.; Kramer, Marianne C.; Gosai, Sager J.; Liu, Xiang; Vandivier, Lee E.; Nelson, Andrew D.L.; Anderson, Zachary D.; Beilstein, Mark A.; Fray, Rupert G.; Lyons, Eric; Gregory, Brian D.

N6-methyladenosine inhibits local ribonucleolytic cleavage to stabilise mRNAs in Arabidopsis Thumbnail


Authors

Stephen J. Anderson

Marianne C. Kramer

Sager J. Gosai

Xiang Liu

Lee E. Vandivier

Andrew D.L. Nelson

Zachary D. Anderson

Mark A. Beilstein

RUPERT FRAY RUPERT.FRAY@NOTTINGHAM.AC.UK
Professor of Epitranscriptomics

Eric Lyons

Brian D. Gregory



Abstract

N6-methyladenosine (m6A) is a dynamic, reversible, covalently modified ribonucleotide that occurs predominantly toward 30 ends of eukaryotic mRNAs and is essential for their proper function and regulation. In Arabidopsis thaliana, many RNAs contain at least one m6A site, yet the transcriptome-wide function of m6A remains mostly unknown. Here, we show that manym6A-modified mRNAs in Arabidopsis have reduced abundance in the absence of this mark. The decrease in abundance is due to transcript destabilization caused by cleavage occurring 4 or 5 nt directly upstream of unmodified m6A sites. Importantly, we also find that, upon agriculturally relevant salt treatment, m6A is dynamically deposited on and stabilizes transcripts encoding proteins required for salt and osmotic stress response. Overall, our findings reveal that m6A generally acts as a stabilizing mark through inhibition of site-specific cleavage in plant transcriptomes, and this mechanism is required for proper regulation of the salt-stress-responsive transcriptome.

Citation

Anderson, S. J., Kramer, M. C., Gosai, S. J., Liu, X., Vandivier, L. E., Nelson, A. D., …Gregory, B. D. (2018). N6-methyladenosine inhibits local ribonucleolytic cleavage to stabilise mRNAs in Arabidopsis. Cell Reports, 25(5), 1146-1157. https://doi.org/10.1016/j.celrep.2018.10.020

Journal Article Type Article
Acceptance Date Oct 3, 2018
Online Publication Date Oct 30, 2018
Publication Date Oct 30, 2018
Deposit Date Oct 24, 2018
Publicly Available Date Oct 24, 2018
Journal Cell Reports
Print ISSN 2211-1247
Publisher Cell Press
Peer Reviewed Peer Reviewed
Volume 25
Issue 5
Pages 1146-1157
DOI https://doi.org/10.1016/j.celrep.2018.10.020
Public URL https://nottingham-repository.worktribe.com/output/1185574
Publisher URL https://www.sciencedirect.com/science/article/pii/S2211124718315961?via%3Dihub

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