Postinfective bowel dysfunction following Campylobacter enteritis is characterised by reduced microbiota diversity and impaired microbiota recovery

Jalanka, Jonna; Gunn, David; Singh, Gulzar; Krishnasamy, Shanthi; Lingaya, Melanie; Crispie, Fiona; Finnegan, Laura; Cotter, Paul; James, Louise; Nowak, Adam; Major, Giles; Spiller, Robin C.

Abstract

Objectives: Persistent bowel dysfunction following gastroenteritis (postinfectious (PI)-BD) is well recognised, but the associated changes in microbiota remain unclear. Our aim was to define these changes after gastroenteritis caused by a single organism, Campylobacter jejuni, examining the dynamic changes in the microbiota and the impact of antibiotics. Design: A single-centre cohort study of 155 patients infected with Campylobacter jejuni. Features of the initial illness as well as current bowel symptoms and the intestinal microbiota composition were recorded soon after infection (visit 1, <40 days) as well as 40-60 days and >80 days later (visits 2 and 3). Microbiota were assessed using 16S rRNA sequencing. Results: PI-BD was found in 22 of the 99 patients who completed the trial. The cases reported significantly looser stools, with more somatic and gastrointestinal symptoms. Microbiota were assessed in 22 cases who had significantly lower diversity and altered microbiota composition compared with the 44 age-matched and sex-matched controls. Moreover 60 days after infection, cases showed a significantly lower abundance of 23 taxa including phylum Firmicutes, particularly in the order Clostridiales and the family Ruminoccocaceae, increased Proteobacteria abundance and increased levels of Fusobacteria and Gammaproteobacteria. The microbiota changes were linked with diet; higher fibre consumption being associated with lower levels of Gammaproteobacteria. Conclusion: The microbiota of PI-BD patients appeared more disturbed by the initial infection compared with the microbiota of those who recovered. The prebiotic effect of high fibre diets may inhibit some of the disturbances seen in PI-BD. Trial registration number: NCT02040922.

Citation

Jalanka, J., Gunn, D., Singh, G., Krishnasamy, S., Lingaya, M., Crispie, F., Finnegan, L., Cotter, P., James, L., Nowak, A., Major, G., & Spiller, R. C. (2022). Postinfective bowel dysfunction following Campylobacter enteritis is characterised by reduced microbiota diversity and impaired microbiota recovery. Gut, https://doi.org/10.1136/gutjnl-2021-326828

• Journal Article Type: Article
• Acceptance Date: Jul 14, 2022
• Online Publication Date: Sep 28, 2022
• Publication Date: Sep 28, 2022
• Deposit Date: Oct 2, 2022
• Publicly Available Date: Oct 3, 2022
• Journal: Gut
• Print ISSN: 0017-5749
• Electronic ISSN: 1468-3288
• Publisher: BMJ Publishing Group
• Peer Reviewed: Peer Reviewed
• DOI: https://doi.org/10.1136/gutjnl-2021-326828
• Keywords: Gastroenterology
• Public URL: https://nottingham-repository.worktribe.com/output/11756156
• Publisher URL: https://gut.bmj.com/content/early/2022/09/28/gutjnl-2021-326828