The reliability of methods to estimate the number and size of human motor units and their use with large limb muscles

Piasecki, M.; Ireland, A.; Piasecki, J.; Stashuk, D.W.; McPhee, J.S.; Jones, D.A.

Abstract

Purpose

Current methods for estimating muscle motor unit (MU) number provide values which are remarkably similar for muscles of widely differing size, probably because surface electrodes sample from similar and relatively small volumes in each muscle. We have evaluated an alternative means of estimating MU number that takes into account differences in muscle size.

Methods

Intramuscular motor unit potentials (MUPs) were recorded and muscle cross-sectional area (CSA) was measured using MRI to provide a motor unit number estimate (iMUNE). This was compared to the traditional MUNE method, using compound muscle action potentials (CMAP) and surface motor unit potentials (sMUPs) recorded using surface electrodes. Data were collected from proximal and distal regions of the vastus lateralis (VL) in young and old men while test–retest reliability was evaluated with VL, tibialis anterior and biceps brachii.

Results

MUPs, sMUPs and CMAPs were highly reliable (r = 0.84–0.91). The traditional MUNE, based on surface recordings, did not differ between proximal and distal sites of the VL despite the proximal CSA being twice the distal CSA. iMUNE, however, gave values that differed between young and old and were proportional to the muscle size.

Conclusion

When evaluating the contribution that MU loss makes to muscle atrophy, such as in disease or ageing, it is important to have a method such as iMUNE, which takes into account any differences in total muscle size.

Citation

Piasecki, M., Ireland, A., Piasecki, J., Stashuk, D., McPhee, J., & Jones, D. (2018). The reliability of methods to estimate the number and size of human motor units and their use with large limb muscles. European Journal of Applied Physiology, 118(4), 767-775. https://doi.org/10.1007/s00421-018-3811-5

• Journal Article Type: Article
• Acceptance Date: Jan 17, 2018
• Online Publication Date: Jan 22, 2018
• Publication Date: 2018-04
• Deposit Date: Oct 16, 2018
• Publicly Available Date: Oct 16, 2018
• Journal: European Journal of Applied Physiology
• Print ISSN: 1439-6319
• Electronic ISSN: 1439-6327
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 118
• Issue: 4
• Pages: 767-775
• DOI: https://doi.org/10.1007/s00421-018-3811-5
• Keywords: Motor unit number estimation; Electromyography; Skeletal muscle; Vastus lateralis
• Public URL: https://nottingham-repository.worktribe.com/output/1167886
• Publisher URL: https://link.springer.com/article/10.1007%2Fs00421-018-3811-5
• Contract Date: Oct 16, 2018