Skip to main content

Research Repository

Advanced Search

H-IPSE is a pathogen-secreted host nucleus-infiltrating protein (Infiltrin) expressed exclusively by the Schistosoma haematobium egg stage

Pennington, Luke F.; Alouffi, Abdulaziz; Mbanefo, Evaristus C.; Ray, Debalina; Heery, David M.; Jardetzky, Theodore S.; Hsieh, Michael H.; Falcone, Franco H.

Authors

Luke F. Pennington

Abdulaziz Alouffi

Evaristus C. Mbanefo

Debalina Ray

DAVID HEERY david.heery@nottingham.ac.uk
Professor of Eucaryoticgene Regulation

Theodore S. Jardetzky

Michael H. Hsieh

Franco H. Falcone



Abstract

Urogenital schistosomiasis, caused by the parasitic trematode Schistosoma haematobium, affects over 112 million people worldwide. As with Schistosoma mansoni infections, the pathology of urogenital schistosomiasis is related mainly to the egg stage, which induces granulomatous inflammation of affected tissues. Schistosoma eggs and their secretions have been studied extensively for the related organism S. mansoni, which is more amenable to laboratory studies. Indeed, we have shown that IPSE/alpha-1 (here M-IPSE), a major protein secreted from S. mansoni eggs, can infiltrate host cells. Although the function of M-IPSE is unknown, its ability to translocate to the nuclei of host cells and bind DNA suggests a possible role in immune modulation of host cell tissues. Whether IPSE homologs are expressed in other schistosome species has not been investigated. Here, we describe the cloning of two paralog genes, H03-IPSE and H06-IPSE, which are orthologs of M-IPSE, from egg cDNA of S. haematobium. Using PCR and immunodetection, we confirmed that the expression of these genes is restricted to the egg stage and female adult worms, while the H-IPSE protein is detectable only in mature eggs and not adults. We show that both H03-IPSE and H06-IPSE proteins can infiltrate HTB-9 bladder cells when added exogenously to culture medium. Monopartite C-terminal nuclear localization sequence (NLS) motifs conserved in H03-IPSE, SKRRRKY, and H06-IPSE SKRGRKY, are responsible for targeting the proteins to the nucleus of HTB-9 cells, as demonstrated by site-directed mutagenesis and green fluorescent protein (GFP) tagging. Thus, S. haematobium eggs express IPSE homologs that appear to perform similar functions in infiltrating host cells.

Journal Article Type Article
Publication Date Dec 29, 2017
Journal Infection and Immunity
Print ISSN 0019-9567
Electronic ISSN 1098-5522
Publisher American Society for Microbiology
Peer Reviewed Peer Reviewed
Volume 85
Issue 12
Article Number e00301-17
APA6 Citation Pennington, L. F., Alouffi, A., Mbanefo, E. C., Ray, D., Heery, D. M., Jardetzky, T. S., …Falcone, F. H. (2017). H-IPSE is a pathogen-secreted host nucleus-infiltrating protein (Infiltrin) expressed exclusively by the Schistosoma haematobium egg stage. Infection and Immunity, 85(12), https://doi.org/10.1128/iai.00301-17
DOI https://doi.org/10.1128/iai.00301-17
Keywords Immunology; Microbiology; Parasitology; Infectious Diseases
Publisher URL https://iai.asm.org/content/85/12/e00301-17

Files





You might also like



Downloadable Citations

;