A double-blind placebo controlled trial into the impacts of HMB supplementation and exercise on free-living muscle protein synthesis, muscle mass and function, in older adults

Din, U.S.U.; Brook, M.S.; Selby, Anna L.; Quinlan, Jonathan I.; Boereboom, Catherine; Abdullah, H.; Franchi, M.; Narici, Marco Vincenzo; Phillips, Bethan E.; Williams, J.W.; Rathmacher, John A.; Wilkinson, D.J.; Atherton, Philip J.; Smith, K.

doi:10.1016/j.clnu.2018.09.025

A double-blind placebo controlled trial into the impacts of HMB supplementation and exercise on free-living muscle protein synthesis, muscle mass and function, in older adults

Din, U.S.U.; Brook, M.S.; Selby, Anna L.; Quinlan, Jonathan I.; Boereboom, Catherine; Abdullah, H.; Franchi, M.; Narici, Marco Vincenzo; Phillips, Bethan E.; Williams, J.W.; Rathmacher, John A.; Wilkinson, D.J.; Atherton, Philip J.; Smith, K.

Authors

U.S.U. Din

MATTHEW BROOK MATTHEW.BROOK@NOTTINGHAM.AC.UK
Assistant Professor

Anna L. Selby

Jonathan I. Quinlan

Catherine Boereboom

H. Abdullah

M. Franchi

Marco Vincenzo Narici

BETH PHILLIPS beth.phillips@nottingham.ac.uk
Professor of Translational Physiology

JOHN WILLIAMS john.williams7@nottingham.ac.uk
Clinical Associate Professor

John A. Rathmacher

DANIEL WILKINSON DANIEL.WILKINSON@NOTTINGHAM.AC.UK
Assistant Professor

PHILIP ATHERTON philip.atherton@nottingham.ac.uk
Professor of Clinical, metabolic & Molecular Physiology

KENNETH SMITH KEN.SMITH@NOTTINGHAM.AC.UK
Professor of Metabolic Mass Spectrometry

Abstract

Age-related sarcopenia and dynapenia are associated with frailty and metabolic diseases. Resistance exercise training (RET) adjuvant to evidence-based nutritional intervention(s) have been shown as mitigating strategies. Given that HMB supplementation during RET improves lean body mass in younger humans (Wilson et al., 2014), and that we have shown that HMB acutely stimulates muscle protein synthesis (MPS) and inhibits breakdown; we hypothesized that chronic supplementation of HMB adjuvant to RET would enhance MPS and muscle mass/function in older people.We recruited 16 healthy older men (PLA: 68.5±1.0y, HMB: 67.8±1.1y) for a randomised double-blind-placebo (PLA) controlled trial (HMB 3 x 1g/day vs. PLA) involving a 6-week unilateral progressive RET regime (6 x 8 repetitions, 75% 1-RM, 3.wk-1). D2O dosing was performed over the first two weeks (0-2w) and last two weeks (4-6w) with bilateral vastus lateralis (VL) biopsies at 0-2 and 4-6 weeks (each time 75±2 min after a single bout of RE) for quantification of early and later MPS responses and post-RE myogenic gene expression. Thigh lean mass was measured by DXA, VL thickness and architecture (fibre length and pennation angle) by ultrasound at 0/3/6 weeks, and strength by knee extensor 1-RM testing and MVC by isokinetic dynamometry (approx. every 10 days).RET induced strength increases (1-RM) in the exercised leg in both groups (398±22N to 499±30N HMB vs. 396±29N to 510±43N PLA (both P

Citation

Din, U., Brook, M., Selby, A. L., Quinlan, J. I., Boereboom, C., Abdullah, H., …Smith, K. (2019). A double-blind placebo controlled trial into the impacts of HMB supplementation and exercise on free-living muscle protein synthesis, muscle mass and function, in older adults. Clinical Nutrition, 38(5), 2071-2078. https://doi.org/10.1016/j.clnu.2018.09.025

Journal Article Type	Article
Acceptance Date	Sep 24, 2018
Online Publication Date	Sep 27, 2018
Publication Date	Oct 31, 2019
Deposit Date	Oct 4, 2018
Publicly Available Date	Dec 10, 2018
Journal	Clinical Nutrition
Print ISSN	0261-5614
Electronic ISSN	1532-1983
Publisher	Elsevier
Peer Reviewed	Peer Reviewed
Volume	38
Issue	5
Pages	2071-2078
DOI	https://doi.org/10.1016/j.clnu.2018.09.025
Keywords	Skeletal muscle; HMB; stable isotopes
Public URL	https://nottingham-repository.worktribe.com/output/1146311
Publisher URL	https://www.sciencedirect.com/science/article/pii/S0261561418324634