A double-blind placebo controlled trial into the impacts of HMB supplementation and exercise on free-living muscle protein synthesis, muscle mass and function, in older adults

Din, U.S.U.; Brook, M.S.; Selby, Anna L.; Quinlan, Jonathan I.; Boereboom, Catherine; Abdullah, H.; Franchi, M.; Narici, Marco Vincenzo; Phillips, Bethan E.; Williams, J.W.; Rathmacher, John A.; Wilkinson, D.J.; Atherton, Philip J.; Smith, K.

Abstract

Age-related sarcopenia and dynapenia are associated with frailty and metabolic diseases. Resistance exercise training (RET) adjuvant to evidence-based nutritional intervention(s) have been shown as mitigating strategies. Given that HMB supplementation during RET improves lean body mass in younger humans (Wilson et al., 2014), and that we have shown that HMB acutely stimulates muscle protein synthesis (MPS) and inhibits breakdown; we hypothesized that chronic supplementation of HMB adjuvant to RET would enhance MPS and muscle mass/function in older people.We recruited 16 healthy older men (PLA: 68.5±1.0y, HMB: 67.8±1.1y) for a randomised double-blind-placebo (PLA) controlled trial (HMB 3 x 1g/day vs. PLA) involving a 6-week unilateral progressive RET regime (6 x 8 repetitions, 75% 1-RM, 3.wk-1). D2O dosing was performed over the first two weeks (0-2w) and last two weeks (4-6w) with bilateral vastus lateralis (VL) biopsies at 0-2 and 4-6 weeks (each time 75±2 min after a single bout of RE) for quantification of early and later MPS responses and post-RE myogenic gene expression. Thigh lean mass was measured by DXA, VL thickness and architecture (fibre length and pennation angle) by ultrasound at 0/3/6 weeks, and strength by knee extensor 1-RM testing and MVC by isokinetic dynamometry (approx. every 10 days).RET induced strength increases (1-RM) in the exercised leg in both groups (398±22N to 499±30N HMB vs. 396±29N to 510±43N PLA (both P

Citation

Din, U., Brook, M., Selby, A. L., Quinlan, J. I., Boereboom, C., Abdullah, H., Franchi, M., Narici, M. V., Phillips, B. E., Williams, J., Rathmacher, J. A., Wilkinson, D., Atherton, P. J., & Smith, K. (2019). A double-blind placebo controlled trial into the impacts of HMB supplementation and exercise on free-living muscle protein synthesis, muscle mass and function, in older adults. Clinical Nutrition, 38(5), 2071-2078. https://doi.org/10.1016/j.clnu.2018.09.025

• Journal Article Type: Article
• Acceptance Date: Sep 24, 2018
• Online Publication Date: Sep 27, 2018
• Publication Date: Oct 31, 2019
• Deposit Date: Oct 4, 2018
• Publicly Available Date: Dec 10, 2018
• Journal: Clinical Nutrition
• Print ISSN: 0261-5614
• Electronic ISSN: 1532-1983
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 38
• Issue: 5
• Pages: 2071-2078
• DOI: https://doi.org/10.1016/j.clnu.2018.09.025
• Keywords: Skeletal muscle; HMB; stable isotopes
• Public URL: https://nottingham-repository.worktribe.com/output/1146311
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0261561418324634
• Contract Date: Oct 4, 2018