Reactive DESI-MS imaging of biological tissues with dicationic ion-pairing compounds

Lostun, Dragos; Perez, Consuelo J.; Licence, Peter; Barrett, David A.; Ifa, Demian R.

Abstract

This work illustrates reactive desorption electrospray ionization mass spectrometry (DESI-MS) with a stable dication on biological tissues. Rat brain and zebra fish tissues were investigated with reactive DESI-MS in which the dictation forms a stable bond with biological tissue fatty acids and lipids. Tandem mass spectrometry (MS/MS) was used to characterize the dication (DC9) and to identify linked lipid-dication compounds formed. The fragment m/z 85 common to both DC9 fragmentation and DC9-lipid fragmentation was used to confirm that DC9 is indeed bonded with the lipids. Lipid signals in the range of m/z 250–350 and phosphoethanolamines (PE) m/z 700–800 observed in negative ion mode were also detected in positive ion mode with reactive DESI-MS with enhanced signal intensity. Reactive DESI-MS imaging in positive ion mode of rat brain and zebra fish tissues allowed enhanced detection of compounds commonly observed in the negative ion mode.

Citation

Lostun, D., Perez, C. J., Licence, P., Barrett, D. A., & Ifa, D. R. (2015). Reactive DESI-MS imaging of biological tissues with dicationic ion-pairing compounds. Analytical Chemistry, 87(6), 3286-3293. https://doi.org/10.1021/ac5042445

• Journal Article Type: Article
• Acceptance Date: Feb 12, 2015
• Online Publication Date: Feb 24, 2015
• Publication Date: Feb 24, 2015
• Deposit Date: Aug 8, 2018
• Print ISSN: 0003-2700
• Electronic ISSN: 1520-6882
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 87
• Issue: 6
• Pages: 3286-3293
• DOI: https://doi.org/10.1021/ac5042445
• Public URL: https://nottingham-repository.worktribe.com/output/1110601
• Publisher URL: https://pubs.acs.org/doi/10.1021/ac5042445
• Related Public URLs: http://www.ncbi.nlm.nih.gov/pubmed/25710577