Reactive DESI-MS imaging of biological tissues with dicationic ion-pairing compounds
Lostun, Dragos; Perez, Consuelo J.; Licence, Peter; Barrett, David A.; Ifa, Demian R.
Consuelo J. Perez
Prof PETER LICENCE PETER.LICENCE@NOTTINGHAM.AC.UK
Professor of Chemistry
David A. Barrett
Demian R. Ifa
This work illustrates reactive desorption electrospray ionization mass spectrometry (DESI-MS) with a stable dication on biological tissues. Rat brain and zebra fish tissues were investigated with reactive DESI-MS in which the dictation forms a stable bond with biological tissue fatty acids and lipids. Tandem mass spectrometry (MS/MS) was used to characterize the dication (DC9) and to identify linked lipid-dication compounds formed. The fragment m/z 85 common to both DC9 fragmentation and DC9-lipid fragmentation was used to confirm that DC9 is indeed bonded with the lipids. Lipid signals in the range of m/z 250–350 and phosphoethanolamines (PE) m/z 700–800 observed in negative ion mode were also detected in positive ion mode with reactive DESI-MS with enhanced signal intensity. Reactive DESI-MS imaging in positive ion mode of rat brain and zebra fish tissues allowed enhanced detection of compounds commonly observed in the negative ion mode.
Lostun, D., Perez, C. J., Licence, P., Barrett, D. A., & Ifa, D. R. (2015). Reactive DESI-MS imaging of biological tissues with dicationic ion-pairing compounds. Analytical Chemistry, 87(6), 3286-3293. doi:10.1021/ac5042445
|Journal Article Type||Article|
|Acceptance Date||Feb 12, 2015|
|Online Publication Date||Feb 24, 2015|
|Publication Date||Feb 24, 2015|
|Deposit Date||Aug 8, 2018|
|Publisher||American Chemical Society|
|Peer Reviewed||Peer Reviewed|