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Impact of extraneous proteins on the gastrointestinal fate of sunflower seed (Helianthus annuus) oil bodies: a simulated gastrointestinal tract study

Makkhun, Sakunkhun; Khosla, Amit; Foster, Tim; McClements, David Julian; Grundy, Myriam M.; Gray, David

Authors

Sakunkhun Makkhun

Amit Khosla

Tim Foster

David Julian McClements

Myriam M. Grundy

DAVID GRAY david.gray@nottingham.ac.uk
Professor of Applied Lipid Science



Abstract

In this study, we examined the physicochemical nature of sunflower seed oil bodies (in the absence and presence of added protein) exposed to gastrointestinal conditions in vitro: crude oil bodies (COB); washed oil bodies (WOB); whey protein isolate-enriched oil bodies (WOB-WPI); and, sodium caseinate enriched-oil bodies (WOB-SC). All oil body emulsions were passed through an in vitro digestion model that mimicked the stomach and duodenal environments, and their physicochemical properties were measured before, during, and after digestion. Oil bodies had a positive charge under gastric conditions because the pH was below the isoelectric point of the adsorbed protein layer, but they had a negative charge under duodenal conditions which was attributed to changes in interfacial composition resulting from adsorption of bile salts. Oil bodies were highly susceptible to flocculation and coalescence in both gastric and duodenal conditions. SDS-PAGE analysis indicated degradation of oleosin proteins (ca. 18–21 kDa) to a greater or lesser extent (dependent on the emulsion) during the gastric phase in all emulsions tested; there is evidence that some oleosin remained intact in the crude oil body preparation during this phase of the digestion process. Measurements of protein displacement from the surface of COBs during direct exposure to bile salts, without inclusion of a gastric phase, indicated the removal of intact oleosin from native oil bodies.

Journal Article Type Article
Acceptance Date Sep 3, 2014
Online Publication Date Sep 4, 2014
Publication Date Jan 1, 2015
Deposit Date Jul 27, 2018
Print ISSN 2042-6496
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 6
Issue 1
Article Number 125-134
DOI https://doi.org/10.1039/C4FO00422A
Public URL https://nottingham-repository.worktribe.com/output/1109324
Publisher URL https://pubs.rsc.org/en/Content/ArticleLanding/2015/FO/C4FO00422A#!divAbstract