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A detailed assessment of varying ejection rate on delivery efficiency of mesenchymal stem cells using narrow-bore needles

Rose, Felicity; Shakesheff, Kevin; White, Lisa; Amer, Mahetab

Authors

Profile image of FELICITY ROSE

FELICITY ROSE FELICITY.ROSE@NOTTINGHAM.AC.UK
Professor of Biomaterials and Tissue Engineering

Kevin Shakesheff

Mahetab Amer



Abstract

As the number of clinical trials exploring cell therapy rises, a thorough understanding of the limits of cell delivery is essential. We used an extensive toolset comprising various standard and multiplex assays for the assessment of cell delivery postejection. Primary human mesenchymal stem cell (hMSC) suspensions were drawn up into 100‐µl Hamilton syringes with 30‐ and 34‐gauge needles attached, before being ejected at rates ranging from 10 to 300 µl/minute. Effects of ejection rate, including changes in viability, apoptosis, senescence, and other key aspects of cellular health, were evaluated. Ejections at slower flow rates resulted in a lower percentage of the cell dose being delivered, and apoptosis measurements of samples ejected at 10 µl/minute were significantly higher than control samples. Immunophenotyping also revealed significant downregulation of CD105 expression in samples ejected at 10 µl/minute (p < .05). Differentiation of ejected hMSCs was investigated using qualitative markers of adipogenesis, osteogenesis, and chondrogenesis, which revealed that slower ejection rates exerted a considerable effect upon the differentiation capacity of ejected cells, thereby possibly influencing the success of cell‐based therapies. The findings of this study demonstrate that ejection rate has substantial impact on the percentage of cell dose delivered and cellular health postejection.

Journal Article Type Article
Acceptance Date Nov 23, 2015
Publication Date Mar 1, 2016
Deposit Date Sep 14, 2018
Print ISSN 2157-6564
Electronic ISSN 2157-6580
Publisher Wiley Open Access
Peer Reviewed Peer Reviewed
Volume 5
Issue 3
Pages 366-378
DOI https://doi.org/10.5966/sctm.2015-0208
Public URL https://nottingham-repository.worktribe.com/output/1109283
Publisher URL https://stemcellsjournals.onlinelibrary.wiley.com/doi/full/10.5966/sctm.2015-0208
PMID 00037195
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