Professor FELICITY ROSE FELICITY.ROSE@NOTTINGHAM.AC.UK
PROFESSOR OF BIOMATERIALS AND TISSUE ENGINEERING
Professor FELICITY ROSE FELICITY.ROSE@NOTTINGHAM.AC.UK
PROFESSOR OF BIOMATERIALS AND TISSUE ENGINEERING
Kevin Shakesheff
Dr LISA WHITE LISA.WHITE@NOTTINGHAM.AC.UK
ASSOCIATE PROFESSOR
Mahetab Amer
As the number of clinical trials exploring cell therapy rises, a thorough understanding of the limits of cell delivery is essential. We used an extensive toolset comprising various standard and multiplex assays for the assessment of cell delivery postejection. Primary human mesenchymal stem cell (hMSC) suspensions were drawn up into 100‐µl Hamilton syringes with 30‐ and 34‐gauge needles attached, before being ejected at rates ranging from 10 to 300 µl/minute. Effects of ejection rate, including changes in viability, apoptosis, senescence, and other key aspects of cellular health, were evaluated. Ejections at slower flow rates resulted in a lower percentage of the cell dose being delivered, and apoptosis measurements of samples ejected at 10 µl/minute were significantly higher than control samples. Immunophenotyping also revealed significant downregulation of CD105 expression in samples ejected at 10 µl/minute (p < .05). Differentiation of ejected hMSCs was investigated using qualitative markers of adipogenesis, osteogenesis, and chondrogenesis, which revealed that slower ejection rates exerted a considerable effect upon the differentiation capacity of ejected cells, thereby possibly influencing the success of cell‐based therapies. The findings of this study demonstrate that ejection rate has substantial impact on the percentage of cell dose delivered and cellular health postejection.
Rose, F., Shakesheff, K., White, L., & Amer, M. (2016). A detailed assessment of varying ejection rate on delivery efficiency of mesenchymal stem cells using narrow-bore needles. Stem Cells Translational Medicine, 5(3), 366-378. https://doi.org/10.5966/sctm.2015-0208
Journal Article Type | Article |
---|---|
Acceptance Date | Nov 23, 2015 |
Publication Date | Mar 1, 2016 |
Deposit Date | Sep 14, 2018 |
Print ISSN | 2157-6564 |
Electronic ISSN | 2157-6580 |
Publisher | Wiley Open Access |
Peer Reviewed | Peer Reviewed |
Volume | 5 |
Issue | 3 |
Pages | 366-378 |
DOI | https://doi.org/10.5966/sctm.2015-0208 |
Public URL | https://nottingham-repository.worktribe.com/output/1109283 |
Publisher URL | https://stemcellsjournals.onlinelibrary.wiley.com/doi/full/10.5966/sctm.2015-0208 |
PMID | 00037195 |
Additional Information | eStaffProfile Description: , eStaffProfile Brief Description of Type: |
Printing biohybrid materials for bioelectronic cardio-3D-cellular constructs
(2022)
Journal Article
About Repository@Nottingham
Administrator e-mail: discovery-access-systems@nottingham.ac.uk
This application uses the following open-source libraries:
Apache License Version 2.0 (http://www.apache.org/licenses/)
Apache License Version 2.0 (http://www.apache.org/licenses/)
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2025
Advanced Search