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Investigating the role of toll-like receptors in models of arthritis

Piccinini, Anna M.; PICCININI, ANNA; Williams, Lynn; McCann, Fiona E.; Midwood, Kim S.


Anna M. Piccinini


Lynn Williams

Fiona E. McCann

Kim S. Midwood


Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by persistent synovial inflammation leading to tissue destruction and progressive loss of joint function. Here we describe two methods that can be used to assess the contribution of toll-like receptors (TLRs), and their potential ligands, to RA pathogenesis. We focus on the antigen-induced model of murine arthritis and human synovial tissue explant models. Both enable detection of TLR, and TLR ligand, expression, as well as investigation of the effect of inhibition of these molecules. Each offers a unique insight into disease; with murine models allowing kinetic analysis in live animals and explant models allowing examination of inflamed human tissue, which together can help us to dissect the role of TLRs in the onset and progression of RA.

Journal Article Type Article
Publication Date Jan 1, 2016
Peer Reviewed Peer Reviewed
Volume 1390
Pages 351-381
APA6 Citation Piccinini, A. M., Williams, L., McCann, F. E., & Midwood, K. S. (2016). Investigating the role of toll-like receptors in models of arthritis. Methods in Molecular Biology, 1390, (351-381). doi:10.1007/978-1-4939-3335-8_22
Publisher URL
Additional Information This is a post-peer-review, pre-copyedit version of an article published in Methods in Molecular Biology. The final authenticated version is available online at:


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