Investigating the role of toll-like receptors in models of arthritis

Piccinini, Anna M.; Williams, Lynn; McCann, Fiona E.; Midwood, Kim S.

Abstract

Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by persistent synovial inflammation leading to tissue destruction and progressive loss of joint function. Here we describe two methods that can be used to assess the contribution of toll-like receptors (TLRs), and their potential ligands, to RA pathogenesis. We focus on the antigen-induced model of murine arthritis and human synovial tissue explant models. Both enable detection of TLR, and TLR ligand, expression, as well as investigation of the effect of inhibition of these molecules. Each offers a unique insight into disease; with murine models allowing kinetic analysis in live animals and explant models allowing examination of inflamed human tissue, which together can help us to dissect the role of TLRs in the onset and progression of RA.

Citation

Piccinini, A. M., Williams, L., McCann, F. E., & Midwood, K. S. (2016). Investigating the role of toll-like receptors in models of arthritis. Methods in Molecular Biology, 1390, 351-381. https://doi.org/10.1007/978-1-4939-3335-8_22

• Journal Article Type: Article
• Acceptance Date: Dec 12, 2014
• Publication Date: Jan 1, 2016
• Deposit Date: Sep 21, 2018
• Publicly Available Date: Oct 22, 2018
• Peer Reviewed: Peer Reviewed
• Volume: 1390
• Pages: 351-381
• DOI: https://doi.org/10.1007/978-1-4939-3335-8_22
• Keywords: Toll-like receptor; Endogenous ligands; DAMPs; sterile inflammation; Antigen-induced arthritis; Human synovial tissue; Rheumatoid arthritis
• Public URL: http://www.ncbi.nlm.nih.gov/pubmed/26803640
• Publisher URL: https://link.springer.com/protocol/10.1007%2F978-1-4939-3335-8_22
• Additional Information: This is a post-peer-review, pre-copyedit version of an article published in Methods in Molecular Biology. The final authenticated version is available online at: http://dx.doi.org/10.1007/978-1-4939-3335-8_22