Plant-influenced gene expression in the rice endophyte Burkholderia kururiensis M130

Coutinho, Bruna G.; Licastro, Danilo; Mendon�a-Previato, Lucia; C�mara, Miguel; Venturi, Vittorio

Abstract

Burkholderia kururiensis M130 is one of the few rice endophytic diazotrophic bacteria identified thus far which is able to enhance growth of rice. To date, very little is known of how strain M130 and other endophytes enter and colonize plants. Here, we identified genes of strain M130 that are differentially regulated in the presence of rice plant extract. A genetic screening of a promoter probe transposon mutant genome bank and RNAseq analysis were performed. The screening of 10,100 insertions of the genomic transposon reporter library resulted in the isolation of 61 insertions displaying differential expression in response to rice macerate. The RNAseq results validated this screen and indicated that this endophytic bacterium undergoes major changes in the presence of plant extract regulating 27.7% of its open reading frames. A large number of differentially expressed genes encode membrane transporters and secretion systems, indicating that the exchange of molecules is an important aspect of bacterial endophytic growth. Genes related to motility, chemotaxis, and adhesion were also overrepresented, further suggesting plant–bacteria interaction. This work highlights the potential close signaling taking place between plants and bacteria and helps us to begin to understand the adaptation of an endophyte in planta

Citation

Coutinho, B. G., Licastro, D., Mendonça-Previato, L., Cámara, M., & Venturi, V. (2015). Plant-influenced gene expression in the rice endophyte Burkholderia kururiensis M130. Molecular Plant-Microbe Interactions, 28(1), 10-21. https://doi.org/10.1094/MPMI-07-14-0225-R

• Journal Article Type: Article
• Acceptance Date: Sep 25, 2014
• Online Publication Date: Jan 31, 2015
• Publication Date: Jan 31, 2015
• Deposit Date: Jun 27, 2018
• Publicly Available Date: Feb 7, 2019
• Journal: Molecular Plant-Microbe Interactions
• Print ISSN: 0894-0282
• Electronic ISSN: 1943-7706
• Publisher: American Phytopathological Society
• Peer Reviewed: Peer Reviewed
• Volume: 28
• Issue: 1
• Pages: 10-21
• DOI: https://doi.org/10.1094/MPMI-07-14-0225-R
• Public URL: https://nottingham-repository.worktribe.com/output/1102201
• Publisher URL: https://apsjournals.apsnet.org/doi/10.1094/MPMI-07-14-0225-R
• PMID: 00034628