Polyionic tags as enhancers of protein solubility in recombinant protein expression

Paraskevopoulou, Vasiliki; Falcone, Franco

Abstract

Since the introduction of recombinant protein expression in the second half of the 1970s, the growth of the biopharmaceutical field has been rapid and protein therapeutics has come to the foreground. Biophysical and structural characterisation of recombinant proteins is the essential prerequisite for their successful development and commercialisation as therapeutics. Despite the challenges, including low protein solubility and inclusion body formation, prokaryotic host systems and particularly Escherichia coli, remain the system of choice for the initial attempt of production of previously unexpressed proteins. Several different approaches have been adopted, including optimisation of growth conditions, expression in the periplasmic space of the bacterial host or co-expression of molecular chaperones, to assist correct protein folding. A very commonly employed approach is also the use of protein fusion tags that enhance protein solubility. Here, a range of experimentally tested peptide tags, which present specific advantages compared to protein fusion tags and the concluding remarks of these experiments are reviewed. Finally, a concept to design solubility-enhancing peptide tags based on a protein's pI is suggested.

Citation

Paraskevopoulou, V., & Falcone, F. (2018). Polyionic tags as enhancers of protein solubility in recombinant protein expression. Microorganisms, 6(2), 47. https://doi.org/10.3390/microorganisms6020047

• Journal Article Type: Review
• Acceptance Date: May 21, 2018
• Online Publication Date: May 23, 2018
• Publication Date: May 23, 2018
• Deposit Date: Oct 26, 2018
• Publicly Available Date: Oct 29, 2018
• Journal: Microorganisms
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 6
• Issue: 2
• Pages: 47
• DOI: https://doi.org/10.3390/microorganisms6020047
• Public URL: https://nottingham-repository.worktribe.com/output/1055105
• Publisher URL: https://www.mdpi.com/2076-2607/6/2/47
• Contract Date: Oct 29, 2018