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Photocrosslinkable gelatin hydrogels modulate the production of the major pro-inflammatory cytokine, TNF-α, by human mononuclear cells

Donaldson, Amy R.; Edi Tanase, Constantin; Awuah, Dennis; Vasanthi Bathri Narayanan, Pranav; Hall, Laurence; Nikkhah, Mehdi; Khademhosseini, Ali; Rose, Felicity; Alexander, Cameron; Ghaemmaghami, Amir M.

Photocrosslinkable gelatin hydrogels modulate the production of the major pro-inflammatory cytokine, TNF-α, by human mononuclear cells Thumbnail


Authors

Amy R. Donaldson

Constantin Edi Tanase

Dennis Awuah

Pranav Vasanthi Bathri Narayanan

Laurence Hall

Mehdi Nikkhah

Ali Khademhosseini



Abstract

Hydrogels are an attractive class of biomaterials in tissue engineering due to their inherently compatible properties for cell culture. Gelatin methacryloyl (GelMA) has shown significant promise in the fields of tissue engineering and drug delivery, as its physical properties can be precisely tuned depending on the specific application. There is a growing appreciation for the interaction between biomaterials and cells of the immune system with the increasing usage of biomaterials for in vivo applications. Here, we addressed the current lack of information regarding the immunogenicity of photocrosslinked GelMA. We investigated the ability of human mononuclear cells to mount inflammatory responses in the context of a GelMA hydrogel platform. Using lipopolysaccharide to stimulate a pro-inflammatory immune response, we found tumour necrosis factor-α (TNF-α) expression was suppressed in GelMA culture conditions. Our findings have important implications on the future use of GelMA and highlight the significance of investigating the potential immune-modulatory properties of biomaterials.

Citation

Donaldson, A. R., Edi Tanase, C., Awuah, D., Vasanthi Bathri Narayanan, P., Hall, L., Nikkhah, M., Khademhosseini, A., Rose, F., Alexander, C., & Ghaemmaghami, A. M. (2018). Photocrosslinkable gelatin hydrogels modulate the production of the major pro-inflammatory cytokine, TNF-α, by human mononuclear cells. Frontiers in Bioengineering and Biotechnology, 6, Article 116. https://doi.org/10.3389/fbioe.2018.00116

Journal Article Type Article
Acceptance Date Jul 27, 2018
Publication Date Sep 19, 2018
Deposit Date Aug 14, 2018
Publicly Available Date Sep 19, 2018
Electronic ISSN 2296-4185
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 6
Article Number 116
DOI https://doi.org/10.3389/fbioe.2018.00116
Keywords Hydrogels, Tumor Necrosis Factors, Immune Modulation, PBMCs, Immune Regulation, Gelatin
Public URL https://nottingham-repository.worktribe.com/output/1031290
Contract Date Sep 19, 2018

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