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Construction of a doxycycline inducible adipogenic lentiviral expression system

Liu, Q.; Hill, P.J.; Karamitri, Angeliki; Ryan, K.J.P.; Chen, H.Y.; Lomax, Michael A.

Authors

Q. Liu

P.J. Hill

Angeliki Karamitri

K.J.P. Ryan

H.Y. Chen

Michael A. Lomax



Abstract

To provide a tool for research on regulating adipocyte differentiation, tetracycline inducible (Tet on) lentiviral expression vectors under the control of an adipose-specific promoter were constructed. The lowest basal expression in the absence of doxycycline and most efficient dose-dependent, doxycycline-induced transient overexpression was observed using vectors constructed with a combination of Tetracycline Responsive Element (TRE) and reverse tetracycline-controlled TransActivator advanced (rtTAadv), transfected in white (3T3-L1) and brown (HIB-1B) preadipocytes cell lines. The results demonstrate that doxycycline adipogenic inducible expression can be achieved using a pLenti TRE / rtTA adv under the control of the truncated aP2 promoter in HIB-1B preadipocytes.

Journal Article Type Article
Publication Date Jan 1, 2013
Journal Plasmid
Print ISSN 0147-619X
Electronic ISSN 0147-619X
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 69
Issue 1
APA6 Citation Liu, Q., Hill, P., Karamitri, A., Ryan, K., Chen, H., & Lomax, M. A. (2013). Construction of a doxycycline inducible adipogenic lentiviral expression system. Plasmid, 69(1), doi:10.1016/j.plasmid.2012.10.001
DOI https://doi.org/10.1016/j.plasmid.2012.10.001
Keywords Tetracycline inducible expression; Lentiviral plasmid; Adipose-specific promoter; Adipocyte
Publisher URL http://www.sciencedirect.com/science/article/pii/S0147619X12001060
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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