Outputs (8)

Impact of neospora caninum infection on the bioenergetics and transcriptome of cerebrovascular endothelial cells (2020)
Journal Article
Elsheikha, H. M., Alkurashi, M., Palfreman, S., Castellanos, M., Kong, K., Ning, E., …MacNaughtan, W. (2020). Impact of neospora caninum infection on the bioenergetics and transcriptome of cerebrovascular endothelial cells. Pathogens, 9(9), Article 710. https://doi.org/10.3390/pathogens9090710

In this work, the effects of the protozoan Neospora caninum on the bioenergetics, chemical composition, and elemental content of human brain microvascular endothelial cells (hBMECs) were investigated. We showed that N. caninum can impair cell mitocho... Read More about Impact of neospora caninum infection on the bioenergetics and transcriptome of cerebrovascular endothelial cells.

Induction and measurement of the early stage of a host-parasite interaction using a combined optical trapping and Raman microspectroscopy system (2019)
Journal Article
Sinjab, F., Elsheikha, H. M., Dooley, M., & Notingher, I. (2020). Induction and measurement of the early stage of a host-parasite interaction using a combined optical trapping and Raman microspectroscopy system. Journal of Biophotonics, 13(2), Article e201960065. https://doi.org/10.1002/jbio.201960065

© 2019 The Authors. Journal of Biophotonics published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Understanding and quantifying the temporal acquisition of host cell molecules by intracellular pathogens is fundamentally important in biology. In t... Read More about Induction and measurement of the early stage of a host-parasite interaction using a combined optical trapping and Raman microspectroscopy system.

Optical sectioning in multi-foci Raman hyperspectral imaging (2018)
Journal Article
Liao, Z., Sinjab, F., Elsheikha, H. M., & Notingher, I. (2018). Optical sectioning in multi-foci Raman hyperspectral imaging. Journal of Raman Spectroscopy, 49(10), 1660-1667. https://doi.org/10.1002/jrs.5450

In this study, we compared the depth-discrimination and speed performance of multi-foci Raman hyperspectral imaging with the reference standard of a single laser point confocal Raman mapping. A liquid crystal spatial light modulator (LC-SLM) was empl... Read More about Optical sectioning in multi-foci Raman hyperspectral imaging.

Holographic optical trapping Raman micro-spectroscopy for interacting live cells (2018)
Preprint / Working Paper
Sinjab, F., Elsheikha, H. M., Awuah, D., Gibson, G., Padgett, M., Ghaemmaghami, A. M., & Notingher, I. Holographic optical trapping Raman micro-spectroscopy for interacting live cells

We present a combined holographic optical tweezers and confocal Raman spectroscopy instrument which allows fast, flexible, and interactive manipulation with molecular measurement of interacting live cell systems. Multiple laser foci created using a s... Read More about Holographic optical trapping Raman micro-spectroscopy for interacting live cells.

Visualizing the interaction of Acanthamoeba castellanii with human retinal epithelial cells by spontaneous Raman and CARS imaging (2017)
Journal Article
Naemat, A., Sinjab, F., McDonald, A., Downes, A., Elfick, A., Elsheikha, H. M., & Notingher, I. (2018). Visualizing the interaction of Acanthamoeba castellanii with human retinal epithelial cells by spontaneous Raman and CARS imaging. Journal of Raman Spectroscopy, 49(3), 412-423. https://doi.org/10.1002/jrs.5296

Improved understanding of the mechanism of nutrient’s uptake can enable targeted manipulation of nutrient sensing pathways in medically important pathogens to a greater degree than is currently possible. In this context, we present the use of spontan... Read More about Visualizing the interaction of Acanthamoeba castellanii with human retinal epithelial cells by spontaneous Raman and CARS imaging.

Tracing amino acid exchange during host-pathogen interaction by combined stable-isotope time-resolved Raman spectral imaging (2016)
Journal Article
Naemat, A., Elsheikha, H. M., Boiter, R., & Notingher, I. (2016). Tracing amino acid exchange during host-pathogen interaction by combined stable-isotope time-resolved Raman spectral imaging. Scientific Reports, 6, Article 20811. https://doi.org/10.1038/srep20811

This study investigates the temporal and spatial interchange of the aromatic amino acid phenylalanine (Phe) between human retinal pigment epithelial cell line (ARPE-19) and tachyzoites of the apicomplexan protozoan parasite Toxoplasma gondii (T. gond... Read More about Tracing amino acid exchange during host-pathogen interaction by combined stable-isotope time-resolved Raman spectral imaging.

Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman spectroscopy (2014)
Journal Article
Naemat, A., Elsheikha, H. M., Al-sandaqchib, A., Kong, K., Ghita, A., & Notingher, I. (2015). Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman spectroscopy. Analyst, 140(3), 756-764. https://doi.org/10.1039/c4an01810a

Label-free imaging using Raman micro-spectroscopy (RMS) was used to characterize the spatio-temporal molecular changes of T. gondii tachyzoites and their host cell microenvironment. Raman spectral maps were recorded from isolated T. gondii tachyzoite... Read More about Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman spectroscopy.

Tracking amino acid’s uptake into the protozoan Acanthamoeba castellanii by stable-isotope labelling and Raman spectral imaging
Presentation / Conference Contribution
Naemat, A., Elsheikha, H. M., & Notingher, I. Tracking amino acid’s uptake into the protozoan Acanthamoeba castellanii by stable-isotope labelling and Raman spectral imaging. Presented at SPIE Photonics Europe, Brussels, Belgium

The capacity of pathogens to acquire nutrients from their host cells is one of the most fundamental aspects of infection biology. Hence, measuring the patterns of nutrients’ uptake by pathogens is essential for understanding the interactions of patho... Read More about Tracking amino acid’s uptake into the protozoan Acanthamoeba castellanii by stable-isotope labelling and Raman spectral imaging.