Outputs (117)

Functional Characterization of Six Eukaryotic Translation Initiation Factors of Toxoplasma gondii Using the CRISPR-Cas9 System (2024)
Journal Article
Kou, Y.-J., Gao, J., Li, R., Ma, Z.-Y., Elsheikha, H. M., Wu, X.-J., Zheng, X.-N., Wang, M., & Zhu, X.-Q. (2024). Functional Characterization of Six Eukaryotic Translation Initiation Factors of Toxoplasma gondii Using the CRISPR-Cas9 System. International Journal of Molecular Sciences, 25(14), Article 7834. https://doi.org/10.3390/ijms25147834

Eukaryotic translation initiation factors (eIFs) are crucial for initiating protein translation and ensuring the correct assembly of mRNA-ribosomal subunit complexes. In this study, we investigated the effects of deleting six eIFs in the apicomplexan... Read More about Functional Characterization of Six Eukaryotic Translation Initiation Factors of Toxoplasma gondii Using the CRISPR-Cas9 System.

Altered landscape of total RNA, tRNA and sncRNA modifications in the liver and spleen of mice infected by Toxoplasma gondii (2024)
Journal Article
Zhang, X.-X., Sun, Y.-Z., Wang, W., Gao, Y., Wei, X.-Y., Sun, H.-C., Wang, C.-R., Ni, H.-B., Yang, X., Elsheikha, H. M., & Guo, H.-P. (2024). Altered landscape of total RNA, tRNA and sncRNA modifications in the liver and spleen of mice infected by Toxoplasma gondii. PLoS Neglected Tropical Diseases, 18(6), Article e0012281. https://doi.org/10.1371/journal.pntd.0012281

Background Pathogens can impact host RNA modification machinery to establish a favorable cellular environment for their replication. In the present study, we investigated the effect of Toxoplasma gondii infection on host RNA modification profiles... Read More about Altered landscape of total RNA, tRNA and sncRNA modifications in the liver and spleen of mice infected by Toxoplasma gondii.

Comparative cytotoxicity of Acanthamoeba castellanii-derived conditioned medium on human corneal epithelial and stromal cells (2024)
Journal Article
Alhazmi, A., Sidney, L. E., Hopkinson, A., & Elsheikha, H. M. (2024). Comparative cytotoxicity of Acanthamoeba castellanii-derived conditioned medium on human corneal epithelial and stromal cells. Acta Tropica, 257, Article 107288. https://doi.org/10.1016/j.actatropica.2024.107288

Soluble factors in the secretome of Acanthamoeba castellanii play crucial roles in the pathogenesis of Acanthamoeba keratitis (AK). Investigating the pathological effects of A. castellanii-derived conditioned medium (ACCM) on ocular cells can provide... Read More about Comparative cytotoxicity of Acanthamoeba castellanii-derived conditioned medium on human corneal epithelial and stromal cells.

Trx4, a novel thioredoxin protein, is important for Toxoplasma gondii fitness (2024)
Journal Article
Zhang, Z.-W., Wang, M., Sun, L.-X., Elsheikha, H. M., Lei, C.-L., Wang, J.-L., …Li, T.-T. (2024). Trx4, a novel thioredoxin protein, is important for Toxoplasma gondii fitness. Parasites and Vectors, 17(1), Article 178. https://doi.org/10.1186/s13071-024-06259-9

Background To successfully replicate within the host cell, Toxoplasma gondii employs several mechanisms to overcome the host cell defenses and mitigate the harmful effects of the free radicals resulting from its own metabolic processes using effector... Read More about Trx4, a novel thioredoxin protein, is important for Toxoplasma gondii fitness.

Global prevalence and risk factors associated with Toxoplasma gondii infection in wild birds: A systematic review and meta-analysis (2024)
Journal Article
Chen, C., Qin, S.-Y., Yang, X., Li, X.-M., Cai, Y., Lei, C.-C., …Cao, H. (2024). Global prevalence and risk factors associated with Toxoplasma gondii infection in wild birds: A systematic review and meta-analysis. Preventive Veterinary Medicine, 226, Article 106187. https://doi.org/10.1016/j.prevetmed.2024.106187

A systematic review and meta-analysis were performed to identify the global prevalence and factors associated with Toxoplasma gondii infection in wild birds. Six bibliographic databases (Chinese National Knowledge Infrastructure, VIP Chinese Journal... Read More about Global prevalence and risk factors associated with Toxoplasma gondii infection in wild birds: A systematic review and meta-analysis.

Expression profiles of host miRNAs and circRNAs and ceRNA network during Toxoplasma gondii lytic cycle (2024)
Journal Article
Wang, S.-S., Wang, X., He, J.-J., Zheng, W.-B., Zhu, X.-Q., Elsheikha, H. M., & Zhou, C.-X. (2024). Expression profiles of host miRNAs and circRNAs and ceRNA network during Toxoplasma gondii lytic cycle. Parasitology Research, 123(2), Article 145. https://doi.org/10.1007/s00436-024-08152-x

Toxoplasma gondii is an opportunistic protozoan parasite that is highly prevalent in the human population and can lead to adverse health consequences in immunocompromised patients and pregnant women. Noncoding RNAs, such as microRNAs (miRNAs) and cir... Read More about Expression profiles of host miRNAs and circRNAs and ceRNA network during Toxoplasma gondii lytic cycle.

An in silico and in vitro assessment of the neurotoxicity of mefloquine (2024)
Journal Article
El Sharazly, B. M., Ahmed, A., Elsheikha, H. M., & Carter, W. G. (2024). An in silico and in vitro assessment of the neurotoxicity of mefloquine. Biomedicines, 12(3), Article 505. https://doi.org/10.3390/biomedicines12030505

Mefloquine (MQ) is a quinoline-based anti-malarial drug used for chemoprophylaxis or as treatment in combination with artesunate. Although MQ has clear anti-Plasmodium properties, it can induce neurotoxicity and undesired neuropsychiatric side effect... Read More about An in silico and in vitro assessment of the neurotoxicity of mefloquine.

The transcription factor AP2XI-2 is a key negative regulator of Toxoplasma gondii merogony (2024)
Journal Article
Wang, J., Li, T., Zhang, N., Wang, M., Sun, L., Zhang, Z., …Zhu, X. (2024). The transcription factor AP2XI-2 is a key negative regulator of Toxoplasma gondii merogony. Nature Communications, 15(1), Article 793. https://doi.org/10.1038/s41467-024-44967-z

Sexual development in Toxoplasma gondii is a multistep process that culminates in the production of oocysts, constituting approximately 50% of human infections. However, the molecular mechanisms governing sexual commitment in this parasite remain poo... Read More about The transcription factor AP2XI-2 is a key negative regulator of Toxoplasma gondii merogony.

Global prevalence of Plasmodium infection in wild birds: A systematic review and meta-analysis (2024)
Journal Article
Yan, W. L., Sun, H. T., Zhao, Y. C., Hou, X. W., Zhang, M., Zhao, Q., …Ni, H. B. (2024). Global prevalence of Plasmodium infection in wild birds: A systematic review and meta-analysis. Research in Veterinary Science, 168, Article 105136. https://doi.org/10.1016/j.rvsc.2024.105136

Avian malaria is a vector-borne parasitic disease caused by Plasmodium infection transmitted to birds by mosquitoes. The aim of this systematic review was to analyze the global prevalence of malaria and risk factors associated with infection in wild... Read More about Global prevalence of Plasmodium infection in wild birds: A systematic review and meta-analysis.

The Toxoplasma protein phosphatase 6 catalytic subunit (TgPP6C) is essential for cell cycle progression and virulence (2023)
Journal Article
Liang, Q. L., Nie, L. B., Elsheikha, H. M., Li, T. T., Sun, L. X., Zhang, Z. W., …Wang, J. L. (2023). The Toxoplasma protein phosphatase 6 catalytic subunit (TgPP6C) is essential for cell cycle progression and virulence. PLoS Pathogens, 19(12), Article e1011831. https://doi.org/10.1371/journal.ppat.1011831

Protein phosphatases are post-translational regulators of Toxoplasma gondii proliferation, tachyzoite-bradyzoite differentiation and pathogenesis. Here, we identify the putative protein phosphatase 6 (TgPP6) subunits of T. gondii and elucidate their... Read More about The Toxoplasma protein phosphatase 6 catalytic subunit (TgPP6C) is essential for cell cycle progression and virulence.