Outputs (188)

Emerging preclinical evidence does not support broad use of hydroxychloroquine in COVID-19 patients (2020)
Journal Article
Funnell, S. G., Dowling, W. E., Muñoz-Fontela, C., Gsell, P. S., Ingber, D. E., Hamilton, G. A., Delang, L., Rocha-Pereira, J., Kaptein, S., Dallmeier, K. H., Neyts, J., Rosenke, K., de Wit, E., Feldmann, H., Maisonnasse, P., Le Grand, R., Frieman, M. B., & Coleman, C. M. (2020). Emerging preclinical evidence does not support broad use of hydroxychloroquine in COVID-19 patients. Nature Communications, 11(1), https://doi.org/10.1038/s41467-020-17907-w

Optical Mapping of cAMP Signaling at the Nanometer Scale (2020)
Journal Article
Bock, A., Annibale, P., Konrad, C., Hannawacker, A., Anton, S. E., Maiellaro, I., …Lohse, M. J. (2020). Optical Mapping of cAMP Signaling at the Nanometer Scale. Cell, 182(6), 1519-1530.e17. https://doi.org/10.1016/j.cell.2020.07.035

Cells relay a plethora of extracellular signals to specific cellular responses by using only a few second messengers, such as cAMP. To explain signaling specificity, cAMP-degrading phosphodiesterases (PDEs) have been suggested to confine cAMP to dist... Read More about Optical Mapping of cAMP Signaling at the Nanometer Scale.

Broad Anti-coronavirus Activity of Food and Drug Administration-Approved Drugs against SARS-CoV-2 In Vitro and SARS-CoV (2020)
Journal Article
Weston, S., Coleman, C. M., Haupt, R., Logue, J., Matthews, K., Li, Y., Reyes, H. M., Weiss, S. R., & Frieman, M. B. (2020). Broad Anti-coronavirus Activity of Food and Drug Administration-Approved Drugs against SARS-CoV-2 In Vitro and SARS-CoV. Journal of Virology, 94(21), Article 10.1128/jvi.01218-20. https://doi.org/10.1128/jvi.01218-20

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in China at the end of 2019 and has rapidly caused a pandemic, with over 20 million recorded COVID-19 cases in August 2020 (https://covid19.who.int/). There are no FDA-approved anti... Read More about Broad Anti-coronavirus Activity of Food and Drug Administration-Approved Drugs against SARS-CoV-2 In Vitro and SARS-CoV.

Broad anti-coronaviral activity of FDA approved drugs against SARS-CoV-2 in vitro and SARS-CoV in vivo. (2020)
Journal Article
Weston, S., Coleman, C., Haupt, R., Logue, J., Matthews, K., Li, Y., …Frieman, M. (2020). Broad anti-coronaviral activity of FDA approved drugs against SARS-CoV-2 in vitro and SARS-CoV in vivo. Journal of Virology, 94(21), Article e01218-20. https://doi.org/10.1128/JVI.01218-20

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in China at the end of 2019 and has rapidly caused a pandemic, with over 20 million recorded COVID-19 cases in August 2020 (https://covid19.who.int/). There are no FDA-approved anti... Read More about Broad anti-coronaviral activity of FDA approved drugs against SARS-CoV-2 in vitro and SARS-CoV in vivo..

Mechanistic insights into Lhr helicase function in DNA repair (2020)
Journal Article
Buckley, R., Kramm, K., Cooper, C. D. O., Grohmann, D., & Bolt, E. L. (2020). Mechanistic insights into Lhr helicase function in DNA repair. Biochemical Journal, 477(16), 2935–2947. https://doi.org/10.1042/bcj20200379

The DNA helicase Lhr is present throughout archaea, including in the Asgard and Nanoarchaea, and has homologues in bacteria and eukaryotes. It is thought to function in DNA repair but in a context that is not known. Our data show that archaeal Lhr pr... Read More about Mechanistic insights into Lhr helicase function in DNA repair.

Widefield, Spatiotemporal Mapping of Spontaneous Activity of Mouse Cultured Neuronal Networks Using Quantum Diamond Sensors (2020)
Journal Article
Price, J. C., Mesquita-Ribeiro, R., Dajas-Bailador, F., & Mather, M. L. (2020). Widefield, Spatiotemporal Mapping of Spontaneous Activity of Mouse Cultured Neuronal Networks Using Quantum Diamond Sensors. Frontiers in Physics, 8, Article 255. https://doi.org/10.3389/fphy.2020.00255

Quantum diamond sensors containing Nitrogen Vacancy (NV) centers were integrated into the culture of spontaneously electrically active cultures of live mouse primary cortical neurons. Two diamond formats were used enabling extracellular studies of ce... Read More about Widefield, Spatiotemporal Mapping of Spontaneous Activity of Mouse Cultured Neuronal Networks Using Quantum Diamond Sensors.

Using singular perturbation theory to determine kinetic parameters in a non-standard coupled enzyme assay (2020)
Journal Article
Dalwadi, M. P., Orol, D., Walter, F., Minton, N. P., King, J. R., & Kovács, K. (2020). Using singular perturbation theory to determine kinetic parameters in a non-standard coupled enzyme assay. Journal of Mathematical Biology, 81(2), 649-690. https://doi.org/10.1007/s00285-020-01524-8

We investigate how to characterize the kinetic parameters of an aminotransaminase using a non-standard coupled (or auxiliary) enzyme assay, where the peculiarity arises for two reasons. First, one of the products of the auxiliary enzyme is a substrat... Read More about Using singular perturbation theory to determine kinetic parameters in a non-standard coupled enzyme assay.

Adjuvant formulated virus-like particles expressing native-like forms of the Lassa virus envelope surface glycoprotein are immunogenic and induce antibodies with broadly neutralizing activity (2020)
Journal Article
Müller, H., Fehling, S. K., Dorna, J., Urbanowicz, R. A., Oestereich, L., Krebs, Y., …Strecker, T. (2020). Adjuvant formulated virus-like particles expressing native-like forms of the Lassa virus envelope surface glycoprotein are immunogenic and induce antibodies with broadly neutralizing activity. npj Vaccines, 5, Article 71. https://doi.org/10.1038/s41541-020-00219-x

Lassa mammarenavirus (LASV) is a rodent-borne arenavirus endemic to several West African countries. It is the causative agent of human Lassa fever, an acute viral hemorrhagic fever disease. To date, no therapeutics or vaccines against LASV have obtai... Read More about Adjuvant formulated virus-like particles expressing native-like forms of the Lassa virus envelope surface glycoprotein are immunogenic and induce antibodies with broadly neutralizing activity.

PDCD4 regulates axonal growth by translational repression of neurite growth-related genes and is modulated during nerve injury responses (2020)
Journal Article
Di Paolo, A. P., Eastman, G., Mesquita Ribeiro, R., Farias, J., Macklin, A., Kislinger, T., …Sotelo Silveira, J. R. (2020). PDCD4 regulates axonal growth by translational repression of neurite growth-related genes and is modulated during nerve injury responses. RNA, 26(11), 1637-1653. https://doi.org/10.1261/rna.075424.120

© 2020 Di Paolo et al. Programmed cell death 4 (PDCD4) protein is a tumor suppressor that inhibits translation through the mTOR-dependent initiation factor EIF4A, but its functional role and mRNA targets in neurons remain largely unknown. Our work id... Read More about PDCD4 regulates axonal growth by translational repression of neurite growth-related genes and is modulated during nerve injury responses.

Gaussia luciferase as a reporter for quorum sensing in staphylococcus aureus (2020)
Journal Article
Blower, I., Tong, C., Sun, X., Murray, E., Luckett, J., Chan, W., …Hill, P. (2020). Gaussia luciferase as a reporter for quorum sensing in staphylococcus aureus. Sensors, 20(15), 1-12. https://doi.org/10.3390/s20154305

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Gaussia luciferase (GLuc) is a secreted protein with significant potential for use as a reporter of gene expression in bacterial pathogenicity studies. To date there are relatively few example... Read More about Gaussia luciferase as a reporter for quorum sensing in staphylococcus aureus.