Two-Photon Excitation of a Plasmonic Nanoswitch Monitored by Single-Molecule Fluorescence Microscopy

Impellizzeri, Stefania; Simoncelli, Sabrina; Hodgson, Gregory K.; Lanterna, Anabel E.; McTiernan, Christopher D.; Raymo, Fran�isco M.; Aramendia, Pedro F.; Scaiano, Juan. C.

Abstract

Visible‐light excitation of the surface plasmon band of silver nanoplates can effectively localize and concentrate the incident electromagnetic field enhancing the photochemical performance of organic molecules. Herein, the first single‐molecule study of the plasmon‐assisted isomerization of a photochrome‐fluorophore dyad, designed to switch between a nonfluorescent and a fluorescent state in response to the photochromic transformation, is reported. The photochemistry of the switchable assembly, consisting of a photochromic benzooxazine chemically conjugated to a coumarin moiety, is examined in real time with total internal reflection fluorescence microscopy in the presence of silver nanoplates excited with a 633 nm laser. The metallic nanostructures significantly enhance the visible light‐induced performance of the photoconversion, which normally requires ultraviolet excitation. The resulting ring‐open isomer is strongly fluorescent and can also be excited at 633 nm. These stochastic emission events are used to monitor photochromic activation and show quadratic dependence on incident power. The utilization of a single laser wavelength for both photochromic activation and excitation effectively mimics a pseudo two‐colours system.

Citation

Impellizzeri, S., Simoncelli, S., Hodgson, G. K., Lanterna, A. E., McTiernan, C. D., Raymo, F. M., …Scaiano, J. C. (2016). Two-Photon Excitation of a Plasmonic Nanoswitch Monitored by Single-Molecule Fluorescence Microscopy. Chemistry - A European Journal, 22(21), 7281-7287. https://doi.org/10.1002/chem.201600218

• Journal Article Type: Article
• Acceptance Date: Jan 16, 2016
• Online Publication Date: Apr 8, 2016
• Publication Date: May 11, 2016
• Deposit Date: Jun 22, 2020
• Publicly Available Date: Jul 27, 2020
• Journal: Chemistry - A European Journal
• Print ISSN: 0947-6539
• Electronic ISSN: 1521-3765
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 22
• Issue: 21
• Pages: 7281-7287
• DOI: https://doi.org/10.1002/chem.201600218
• Public URL: https://nottingham-repository.worktribe.com/output/4442726
• Publisher URL: https://chemistry-europe.onlinelibrary.wiley.com/doi/abs/10.1002/chem.201600218
• Additional Information: This is the pre-peer reviewed version of the following article S. Impellizzeri, S. Simoncelli, G. K. Hodgson, A. E. Lanterna, C. D. McTiernan, F. M. Raymo, P. F. Aramendia, J. C. Scaiano, Chem. Eur. J. 2016 , 22 , 7281, which has been published in final form at https://doi.org/10.1002/chem.201600218. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.