A novel high sensitivity bacteriophage-based assay identifies low level Mycobacterium tuberculosis bacteraemia in immunocompetent patients with active and incipient tuberculosis

Verma, Raman; Swift, Benjamin M.C.; Handley-Hartill, Wade; Lee, Joanne K.; Woltmann, Gerrit; Rees, Catherine E.D.; Haldar, Pranabashis

Abstract

Haematogenous dissemination of M. tuberculosis (Mtb) is critical to pathogenesis of progressive tuberculous infection in animal models. Using a novel phage-based blood assay, we report the first concordant evidence in well-characterised immunocompetent human cohorts, demonstrating associations of Mtb bacteraemia with progressive phenotypes of latent infection and active pulmonary TB respectively.

Citation

Verma, R., Swift, B. M., Handley-Hartill, W., Lee, J. K., Woltmann, G., Rees, C. E., & Haldar, P. (2020). A novel high sensitivity bacteriophage-based assay identifies low level Mycobacterium tuberculosis bacteraemia in immunocompetent patients with active and incipient tuberculosis. Clinical Infectious Diseases, 70(5), 933-936. https://doi.org/10.1093/cid/ciz548

• Journal Article Type: Article
• Acceptance Date: Jun 20, 2019
• Online Publication Date: Jun 22, 2019
• Publication Date: Mar 1, 2020
• Deposit Date: Aug 8, 2019
• Publicly Available Date: Jun 23, 2020
• Journal: Clinical Infectious Diseases
• Print ISSN: 1058-4838
• Electronic ISSN: 1537-6591
• Publisher: Oxford University Press
• Peer Reviewed: Peer Reviewed
• Volume: 70
• Issue: 5
• Pages: 933-936
• DOI: https://doi.org/10.1093/cid/ciz548
• Keywords: Bacteriophage; Bacteremia; Human tuberculosis; Blood
• Public URL: https://nottingham-repository.worktribe.com/output/2302953
• Publisher URL: https://academic.oup.com/cid/advance-article/doi/10.1093/cid/ciz548/5522421
• Additional Information: This is a pre-copyedited, author-produced version of an article accepted for publication in Clinical Infectious Diseases following peer review. The version of record Raman Verma, Benjamin M C Swift, Wade Handley-Hartill, Joanne K Lee, Gerrit Woltmann, Catherine E D Rees, Pranabashis Haldar, A Novel, High-sensitivity, Bacteriophage-based Assay Identifies Low-level Mycobacterium tuberculosis Bacteremia in Immunocompetent Patients With Active and Incipient Tuberculosis, Clinical Infectious Diseases, , ciz548is available online at: https://doi.org/10.1093/cid/ciz548
• Contract Date: Aug 8, 2019