Ersilia Nigro
An ancestral host defence peptide within human beta-defensin 3 recapitulates the antibacterial and antiviral activity of the full-length molecule
Nigro, Ersilia; Colavita, Irene; Sarnataro, Daniela; Scudiero, Olga; Zambrano, Gerardo; Granata, Vincenzo; Daniele, Aurora; Carotenuto, Alfonso; Galdiero, Stefania; Folliero, Veronica; Galdiero, Massimiliano; Urbanowicz, Richard; Ball, Jonathan; Salvatore, Francesco; Pessi, Antonello
Authors
Irene Colavita
Daniela Sarnataro
Olga Scudiero
Gerardo Zambrano
Vincenzo Granata
Aurora Daniele
Alfonso Carotenuto
Stefania Galdiero
Veronica Folliero
Massimiliano Galdiero
Richard Urbanowicz
JONATHAN BALL jonathan.ball@nottingham.ac.uk
Professor of Molecular Virology
Francesco Salvatore
Antonello Pessi
Abstract
Host defence peptides (HDPs) are critical components of innate immunity. Despite their diversity, they share common features including a structural signature, designated “γ-core motif”. We reasoned that for each HDPs evolved from an ancestral γ-core, the latter should be the evolutionary starting point of the molecule, i.e. it should represent a structural scaffold for the modular construction of the full-length molecule, and possess biological properties. We explored the γ-core of human β-defensin 3 (HBD3) and found that it: (a) is the folding nucleus of HBD3; (b) folds rapidly and is stable in human serum; (c) displays antibacterial activity; (d) binds to CD98, which mediates HBD3 internalization in eukaryotic cells; (e) exerts antiviral activity against human immunodeficiency virus and herpes simplex virus; and (f) is not toxic to human cells. These results demonstrate that the γ-core within HBD3 is the ancestral core of the full-length molecule and is a viable HDP per se,since it is endowed with the most important biological features of HBD3. Notably, the small, stable scaffold of the HBD3 γ-core can be exploited to design disease-specific antimicrobial agents.
Citation
Nigro, E., Colavita, I., Sarnataro, D., Scudiero, O., Zambrano, G., Granata, V., …Pessi, A. (2015). An ancestral host defence peptide within human beta-defensin 3 recapitulates the antibacterial and antiviral activity of the full-length molecule. Scientific Reports, 5, Article 18450. https://doi.org/10.1038/srep18450
Journal Article Type | Article |
---|---|
Acceptance Date | Nov 9, 2015 |
Online Publication Date | Dec 21, 2015 |
Publication Date | Dec 21, 2015 |
Deposit Date | Jun 26, 2018 |
Publicly Available Date | Jan 29, 2019 |
Journal | Scientific Reports |
Publisher | Nature Publishing Group |
Peer Reviewed | Peer Reviewed |
Volume | 5 |
Article Number | 18450 |
DOI | https://doi.org/10.1038/srep18450 |
Public URL | https://nottingham-repository.worktribe.com/output/1114908 |
Publisher URL | https://www.nature.com/articles/srep18450 |
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