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The antioxidant protein glutaredoxin 1 is essential for oxidative stress response and pathogenicity of Toxoplasma gondii (2023)
Journal Article
Li, T.-T., Zhao, D.-Y., Liang, Q.-L., Elsheikha, H. M., Wang, M., Sun, L.-X., Zhang, Z.-W., Chen, X.-Q., Zhu, X.-Q., & Wang, J.-L. (2023). The antioxidant protein glutaredoxin 1 is essential for oxidative stress response and pathogenicity of Toxoplasma gondii. The FASEB Journal, 37(6), Article e22932. https://doi.org/10.1096/fj.202201275r

Glutaredoxins (Grxs) are ubiquitous antioxidant proteins involved in many molecular processes to protect cells against oxidative damage. Here, we study the roles of Grxs in the pathogenicity of Toxoplasma gondii. We show that Grxs are localized in th... Read More about The antioxidant protein glutaredoxin 1 is essential for oxidative stress response and pathogenicity of Toxoplasma gondii.

Unravelling Strain-Specific Modifications of Toxoplasma gondii tRNA and sncRNA Using LC-MS/MS (2023)
Journal Article
Wang, W., Yang, Y., Guo, H., Li, M. H., Chen, X. Q., Wei, X. Y., Chen, Y., Elsheikha, H. M., & Zhang, X. X. (2023). Unravelling Strain-Specific Modifications of Toxoplasma gondii tRNA and sncRNA Using LC-MS/MS. Microbiology Spectrum, 11(3), Article e0356422. https://doi.org/10.1128/spectrum.03564-22

Many RNA modifications have been detected in rRNA, tRNA and small noncoding RNA (sncRNA) as well as in low-abundance RNA species such mRNA. Although RNA modifications play roles in many cellular and biological processes in various domains of life, kn... Read More about Unravelling Strain-Specific Modifications of Toxoplasma gondii tRNA and sncRNA Using LC-MS/MS.

Modulation of long noncoding RNA (lncRNA) and messenger RNA (mRNA) expression in the liver of Beagle dogs by Toxocara canis infection (2023)
Journal Article
Zou, Y., Zheng, W. B., Elsheikha, H. M., He, J. J., Lu, Y. X., Wang, S., Guo, A., & Zhu, X. Q. (2023). Modulation of long noncoding RNA (lncRNA) and messenger RNA (mRNA) expression in the liver of Beagle dogs by Toxocara canis infection. Parasites and Vectors, 16, Article 114. https://doi.org/10.1186/s13071-023-05738-9

Background Long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) play crucial roles in regulating various physiological and pathological processes. However, the role of lncRNAs and mRNAs in mediating the liver response during Toxocara canis infec... Read More about Modulation of long noncoding RNA (lncRNA) and messenger RNA (mRNA) expression in the liver of Beagle dogs by Toxocara canis infection.

Functional Characterization of 15 Novel Dense Granule Proteins in Toxoplasma gondii Using the CRISPR-Cas9 System (2022)
Journal Article
Zheng, X. N., Wang, J. L., Elsheikha, H. M., Wang, M., Zhang, Z. W., Sun, L. X., Wang, X. C., Zhu, X. Q., & Li, T. T. (2023). Functional Characterization of 15 Novel Dense Granule Proteins in Toxoplasma gondii Using the CRISPR-Cas9 System. Microbiology Spectrum, 11(1), https://doi.org/10.1128/spectrum.03078-22

The analysis of the subcellular localization and function of dense granule proteins (GRAs) is of central importance for the understanding of host-parasite interaction and pathogenesis of Toxoplasma gondii infection. Here, we identified 15 novel GRAs... Read More about Functional Characterization of 15 Novel Dense Granule Proteins in Toxoplasma gondii Using the CRISPR-Cas9 System.

The protein phosphatase 2A holoenzyme is a key regulator of starch metabolism and bradyzoite differentiation in Toxoplasma gondii (2022)
Journal Article
Wang, J.-L., Li, T.-T., Elsheikha, H. M., Liang, Q.-L., Zhang, Z.-W., Wang, M., Sibley, L. D., & Zhu, X.-Q. (2022). The protein phosphatase 2A holoenzyme is a key regulator of starch metabolism and bradyzoite differentiation in Toxoplasma gondii. Nature Communications, 13(1), Article 7560. https://doi.org/10.1038/s41467-022-35267-5

Phenotypic switching between tachyzoite and bradyzoite is the fundamental mechanism underpinning the pathogenicity and adaptability of the protozoan parasite Toxoplasma gondii. Although accumulation of cytoplasmic starch granules is a hallmark of the... Read More about The protein phosphatase 2A holoenzyme is a key regulator of starch metabolism and bradyzoite differentiation in Toxoplasma gondii.

Effect of deleting four Toxoplasma gondii calcium-binding EGF domain-containing proteins on parasite replication and virulence (2022)
Journal Article
Wang, X.-C., Li, T.-T., Elsheikha, H. M., Zheng, X.-N., Zhao, D.-Y., Wang, J.-L., Wang, M., & Zhu, X.-Q. (2023). Effect of deleting four Toxoplasma gondii calcium-binding EGF domain-containing proteins on parasite replication and virulence. Parasitology Research, 122, 441-450. https://doi.org/10.1007/s00436-022-07739-6

Several calcium-binding proteins including calcium-dependent protein kinases play important roles in several facets of the intracellular infection cycle of the apicomplexan protozoan parasite Toxoplasma gondii. However, the role of the calcium-bindin... Read More about Effect of deleting four Toxoplasma gondii calcium-binding EGF domain-containing proteins on parasite replication and virulence.

Camellia sinensis solvent extract, epigallocatechin gallate and caffeine confer trophocidal and cysticidal effects against Acanthamoeba castellanii (2022)
Journal Article
Fakae, L. B., Harun, M. S., Ting, D. S. J., Dua, H. S., Cave, G. W., Zhu, X. Q., Stevenson, C. W., & Elsheikha, H. M. (2023). Camellia sinensis solvent extract, epigallocatechin gallate and caffeine confer trophocidal and cysticidal effects against Acanthamoeba castellanii. Acta Tropica, 237, Article 106729. https://doi.org/10.1016/j.actatropica.2022.106729

We examined the anti-acanthamoebic efficacy of green tea Camellia sinensis solvent extract (SE) or its chemical constituents against Acanthamoeba castellanii by using anti-trophozoite, anti-encystation, and anti-excystation assays. C. sinensis SE (62... Read More about Camellia sinensis solvent extract, epigallocatechin gallate and caffeine confer trophocidal and cysticidal effects against Acanthamoeba castellanii.

Microfluidic-Based Formulation of Essential Oils-Loaded Chitosan Coated PLGA Particles Enhances Their Bioavailability and Nematocidal Activity (2022)
Journal Article
Helal, M. A., Abdel-Gawad, A. M., Kandil, O. M., Khalifa, M. M., Morrison, A. A., Bartley, D. J., Cave, G. W., & Elsheikha, H. M. (2022). Microfluidic-Based Formulation of Essential Oils-Loaded Chitosan Coated PLGA Particles Enhances Their Bioavailability and Nematocidal Activity. Pharmaceutics, 14(10), Article 2030. https://doi.org/10.3390/pharmaceutics14102030

In this study, poly (lactic-co-glycolic) acid (PLGA) particles were synthesized and coated with chitosan. Three essential oil (EO) components (eugenol, linalool, and geraniol) were entrapped inside these PLGA particles by using the continuous flow-fo... Read More about Microfluidic-Based Formulation of Essential Oils-Loaded Chitosan Coated PLGA Particles Enhances Their Bioavailability and Nematocidal Activity.

Lipidomic changes in the liver of beagle dogs associated with Toxocara canis infection (2022)
Journal Article
Li, H. Y., Zou, Y., Elsheikha, H. M., Xu, Y., Cai, L., Xie, S. C., Zhu, X. Q., & Zheng, W. B. (2022). Lipidomic changes in the liver of beagle dogs associated with Toxocara canis infection. Frontiers in Cellular and Infection Microbiology, 12, Article 890589. https://doi.org/10.3389/fcimb.2022.890589

A global lipidomic analysis using liquid chromatography–tandem mass spectrometry was performed on the liver of beagle dogs infected with Toxocara canis to profile hepatic lipid species at 12h post-infection (hpi), 24 hpi, and 36 days post-infection (... Read More about Lipidomic changes in the liver of beagle dogs associated with Toxocara canis infection.