Simplified footprint-free Cas9/CRISPR editing of cardiac-associated genes in human pluripotent stem cells
(2018)
Journal Article
Kondrashov, A., Hoang, M. D., Smith, J. G., Bhagwan, J. R., Duncan, G., Mosqueira, D., Barbadillo Munoz, M., Vo, N. T., & Denning, C. (in press). Simplified footprint-free Cas9/CRISPR editing of cardiac-associated genes in human pluripotent stem cells. Stem Cells and Development, 27(6), https://doi.org/10.1089/scd.2017.0268
Modelling disease with hPSCs is hindered because the impact on cell phenotype from genetic variability between individuals can be greater than from the pathogenic mutation. While ‘footprint-free’ Cas9/CRISPR editing solves this issue, existing approa... Read More about Simplified footprint-free Cas9/CRISPR editing of cardiac-associated genes in human pluripotent stem cells.