All Outputs (7)

SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance (2016)
Journal Article
Asiani, K. R., Williams, H. E. L., Bird, L., Jenner, M., Searle, M. S., Hobman, J. L., …Soultanas, P. (2016). SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance. Molecular Microbiology, 101(5), 731-742. https://doi.org/10.1111/mmi.13399

Ag+ resistance was initially found on the Salmonella enetrica serovar Typhimurium multi-resistance plasmid pMG101 from burns patients in 1975. The putative model of Ag+ resistance, encoded by the sil operon from pMG101, involves export of Ag+ via an... Read More about SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance.

Defining the intrinsically disordered C-terminal domain of SSB reveals DNA-mediated compaction (2015)
Journal Article
Green, M., Hatter, L., Brookes, E., Soultanas, P., & Scott, D. (2016). Defining the intrinsically disordered C-terminal domain of SSB reveals DNA-mediated compaction. Journal of Molecular Biology, 428(2), 357-364. https://doi.org/10.1016/j.jmb.2015.12.007

The bacterial single-stranded DNA (ssDNA) binding protein SSB is a strictly conserved and essential protein involved in diverse functions of DNA metabolism, including replication and repair. SSB comprises a well-characterized tetrameric core of N-ter... Read More about Defining the intrinsically disordered C-terminal domain of SSB reveals DNA-mediated compaction.

Engineering a reagentless biosensor for single-stranded DNA to measure real-time helicase activity in Bacillus (2014)
Journal Article
Green, M., Gilhooly, N. S., Abedeen, S., Scott, D. J., Dillingham, M. S., & Soultanas, P. (2014). Engineering a reagentless biosensor for single-stranded DNA to measure real-time helicase activity in Bacillus. Biosensors and Bioelectronics, 61, 579-586. https://doi.org/10.1016/j.bios.2014.06.011

Single-stranded DNA-binding protein (SSB) is a well characterized ubiquitous and essential bacterial protein involved in almost all aspects of DNA metabolism. Using the Bacillus subtilis SSB we have generated a reagentless SSB biosensor that can be u... Read More about Engineering a reagentless biosensor for single-stranded DNA to measure real-time helicase activity in Bacillus.

Enthalpy/entropy compensation effects from cavity desolvation underpin broad ligand binding selectivity for rat odorant binding protein 3 (2014)
Journal Article
Portman, K. L., Long, J., Carr, S., Briand, L., Winzor, D. J., Searle, M. S., & Scott, D. J. (2014). Enthalpy/entropy compensation effects from cavity desolvation underpin broad ligand binding selectivity for rat odorant binding protein 3. Biochemistry, 53(14), 2371-2379. doi:10.1021/bi5002344

The lipidome and proteome of oil bodies from Helianthus annuus (common sunflower) (2013)
Journal Article
Furse, S., Liddell, S., Ortori, C. A., Williams, H., Neylon, D. C., Scott, D. J., …Gray, D. A. (2013). The lipidome and proteome of oil bodies from Helianthus annuus (common sunflower). Journal of Chemical Biology, 6(2), 63-76. https://doi.org/10.1007/s12154-012-0090-1

In this paper we report the molecular profiling, lipidome and proteome, of the plant organelle known as an oil body (OB). The OB is remarkable in that it is able to perform its biological role (storage of triglycerides) whilst resisting the physical... Read More about The lipidome and proteome of oil bodies from Helianthus annuus (common sunflower).

DnaB proteolysis in vivo regulates oligomerization and its localization at oriC in Bacillus subtilis (2010)
Journal Article
Grainger, W. H., Machón, C., Scott, D. J., & Soultanas, P. (2010). DnaB proteolysis in vivo regulates oligomerization and its localization at oriC in Bacillus subtilis. Nucleic Acids Research, 38(9), https://doi.org/10.1093/nar/gkp1236

Initiation of bacterial DNA replication at oriC is mediated by primosomal proteins that act cooperatively to melt an AT-rich region where the replicative helicase is loaded prior to the assembly of the replication fork. In Bacillus subtilis, the dnaD... Read More about DnaB proteolysis in vivo regulates oligomerization and its localization at oriC in Bacillus subtilis.

RepD-mediated recruitment of PcrA helicase at the Staphylococcus aureus pC221 plasmid replication origin, oriD (2010)
Journal Article
Mach?n, C., Thomson, N., Lynch, G., Scott, D. J., Thomas, C., & Soultanas, P. (2010). RepD-mediated recruitment of PcrA helicase at the Staphylococcus aureus pC221 plasmid replication origin, oriD. Nucleic Acids Research, 38(6), https://doi.org/10.1093/nar/gkp1153

Plasmid encoded replication initiation (Rep) proteins recruit host helicases to plasmid replication origins. Previously, we showed that RepD recruits directionally the PcrA helicase to the pC221 oriD, remains associated with it, and increases its pro... Read More about RepD-mediated recruitment of PcrA helicase at the Staphylococcus aureus pC221 plasmid replication origin, oriD.