Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors

Ahmed, B.Y.; Chakravarthy, S.; Eggers, R.; Hermens, W.T.; Zhang, J.Y.; Niclou, S.P.; Levelt, C.; Sablitzky, F.; Anderson, P.N.; Lieberman, A.; Verhaagen, J.

doi:10.1186/1471-2202-5-4

All Outputs (6)

Towards improved butanol production through targeted genetic modification of Clostridium pasteurianum (2017)
Journal Article
Schwarz, K., Grosse-Honebrink, A., Derecka, K., Rotta, C., Zhang, Y., & Minton, N. P. (2017). Towards improved butanol production through targeted genetic modification of Clostridium pasteurianum. Metabolic Engineering, 40, 124-137. https://doi.org/10.1016/j.ymben.2017.01.009

Declining fossil fuel reserves, coupled with environmental concerns over their continued extraction and exploitation have led to strenuous efforts to identify renewable routes to energy and fuels. One attractive option is to convert glycerol, a by-pr... Read More about Towards improved butanol production through targeted genetic modification of Clostridium pasteurianum.

Enhanced solvent production by metabolic engineering of a twin-clostridial consortium (2016)
Journal Article
Wen, Z., Minton, N. P., Zhang, Y., Li, Q., Liu, J., Jiang, Y., & Yang, S. (2017). Enhanced solvent production by metabolic engineering of a twin-clostridial consortium. Metabolic Engineering, 39, 38-48. https://doi.org/10.1016/j.ymben.2016.10.013

CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii (2016)
Journal Article

Solventogenic clostridia are important industrial microorganisms that produce various chemicals and fuels. Effective genetic tools would facilitate physiological studies aimed both at improving our understanding of metabolism and optimizing solvent p... Read More about CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii.

Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum (2016)
Journal Article
Zhang, Y., Xu, S., Chai, C., Yang, S., Jiang, W., Minton, N. P., & Gu, Y. (2016). Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum. FEMS Microbiology Letters, 363(8), Article fnw065. https://doi.org/10.1093/femsle/fnw065

Clostridium acetobutylicum is an industrially important Gram-positive organism which is capable of producing economically important chemicals in the ABE (Acetone, Butanol and Ethanol) fermentation process. Renewed interests in the ABE process necessi... Read More about Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum.

A universal mariner transposon system for forward genetic studies in the genus Clostridium (2015)
Journal Article

DNA transposons represent an essential tool in the armoury of the molecular microbiologist. We previously developed a catP-based mini transposon system for Clostridium difficile in which the expression of the transposase gene was dependent on a sigma... Read More about A universal mariner transposon system for forward genetic studies in the genus Clostridium.

Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors (2004)
Journal Article
Ahmed, B., Chakravarthy, S., Eggers, R., Hermens, W., Zhang, J., Niclou, S., …Verhaagen, J. (2004). Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors. BMC Neuroscience, 5(1), Article 4. https://doi.org/10.1186/1471-2202-5-4

BACKGROUND: Inactivating genes in vivo is an important technique for establishing their function in the adult nervous system. Unfortunately, conventional knockout mice may suffer from several limitations including embryonic or perinatal lethality and... Read More about Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors.