All Outputs (11)

Fully angularly resolved 3D microrheology with optical tweezers (2024)
Journal Article
Matheson, A. B., Mendonca, T., Smith, M. G., Sutcliffe, B., Fernandez, A. J., Paterson, L., …Tassieri, M. (2024). Fully angularly resolved 3D microrheology with optical tweezers. Rheologica Acta, 63(3), 205-217. https://doi.org/10.1007/s00397-024-01435-1

Microrheology with optical tweezers (MOT) is an all-optical technique that allows the user to investigate a materials’ viscoelastic properties at microscopic scales, and is particularly useful for those materials that feature complex microstructures,... Read More about Fully angularly resolved 3D microrheology with optical tweezers.

OptoRheo: Simultaneous in situ micro-mechanical sensing and imaging of live 3D biological systems (2023)
Journal Article
Mendonca, T., Lis-Slimak, K., Matheson, A. B., Smith, M. G., Anane-Adjei, A. B., Ashworth, J. C., …Wright, A. J. (2023). OptoRheo: Simultaneous in situ micro-mechanical sensing and imaging of live 3D biological systems. Communications Biology, 6, Article 463. https://doi.org/10.1038/s42003-023-04780-8

Biomechanical cues from the extracellular matrix (ECM) are essential for directing many cellular processes, from normal development and repair, to disease progression. To better understand cell-matrix interactions, we have developed a new instrument... Read More about OptoRheo: Simultaneous in situ micro-mechanical sensing and imaging of live 3D biological systems.

OptoRheo: Simultaneous in situ micro-mechanical sensing and 3D imaging of live cell cultures (2023)
Preprint / Working Paper
Mendonca, T., Lis-Slimak, K., Matheson, A. B., Smith, M. G., Anane-Adjei, A. B., Cavanagh, R., …Wright, A. J. OptoRheo: Simultaneous in situ micro-mechanical sensing and 3D imaging of live cell cultures

Biomechanical cues from the extracellular matrix (ECM) are essential for directing many cellular processes, from normal development and repair, to disease progression. To better understand cell-matrix interactions, we have developed a new instrument... Read More about OptoRheo: Simultaneous in situ micro-mechanical sensing and 3D imaging of live cell cultures.

Origami: Single-cell 3D shape dynamics oriented along the apico-basal axis of folding epithelia from fluorescence microscopy data (2021)
Journal Article
Mendonca, T., Jones, A. A., Pozo, J. M., Baxendale, S., Whitfield, T. T., & Frangi, A. F. (2021). Origami: Single-cell 3D shape dynamics oriented along the apico-basal axis of folding epithelia from fluorescence microscopy data. PLoS Computational Biology, 17(11), Article e1009063. https://doi.org/10.1371/journal.pcbi.1009063

A common feature of morphogenesis is the formation of three-dimensional structures from the folding of two-dimensional epithelial sheets, aided by cell shape changes at the cellularlevel. Changes in cell shape must be studied in the context of cell-p... Read More about Origami: Single-cell 3D shape dynamics oriented along the apico-basal axis of folding epithelia from fluorescence microscopy data.

Correction to: A trade-off between thickness and length in the zebra finch sperm mid-piece (2021)
Journal Article
Mendonca, T., Birkhead, T. R., Cadby, A. J., Forstmeier, W., & Hemmings, N. (2021). Correction to: A trade-off between thickness and length in the zebra finch sperm mid-piece. Proceedings of the Royal Society B: Biological Sciences, 288(1958), Article 20211695. https://doi.org/10.1098/rspb.2021.1695

The original article contains a typographical mistake in the formula notation for the volume of the sperm mitochondrial helix. In the discussion (paragraph 6), we refer to the formula as 'ν = (1/3)π(r2)T'; however, the formula used in our calculation... Read More about Correction to: A trade-off between thickness and length in the zebra finch sperm mid-piece.

Microrheology With an Anisotropic Optical Trap (2021)
Journal Article
Gibson, G. M., Dalgarno, P. A., Wright, A., Paterson, L., Mendonca, T., Matheson, A. B., & Tassier, M. (2021). Microrheology With an Anisotropic Optical Trap. Frontiers in Physics, 9, Article 621512. https://doi.org/10.3389/fphy.2021.621512

Microrheology with optical tweezers (MOT) measurements are usually performed using optical traps that are close to isotropic across the plane being imaged, but little is known about what happens when this is not the case. In this work, we investigate... Read More about Microrheology With an Anisotropic Optical Trap.

Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM): a new tool for 3D microrheology (2021)
Journal Article
Matheson, A. B., Paterson, L., Wright, A. J., Mendonca, T., Tassieri, M., & Dalgarno, P. A. (2021). Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM): a new tool for 3D microrheology. Scientific Reports, 11, Article 5614. https://doi.org/10.1038/s41598-021-85013-y

We introduce a novel 3D microrheology system that combines for the first time Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM). The system allows the 3D tracking of an optically trapped bead, with ~ 20 nm accuracy along the optical ax... Read More about Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM): a new tool for 3D microrheology.

Microrheology reveals microscale viscosity gradients in planktonic systems (2020)
Journal Article
Guadayol, O., Mendonca, T., Segura-Noguera, M., Wright, A. J., Tassieri, M., & Humphries, S. (2021). Microrheology reveals microscale viscosity gradients in planktonic systems. Proceedings of the National Academy of Sciences, 118(1), Article e2011389118. https://doi.org/10.1073/pnas.2011389118

Microbial activity in planktonic systems creates a dynamic and heterogeneous microscale seascape that harbors a diverse community of microorganisms and ecological interactions of global significance. In recent decades great effort has been put into u... Read More about Microrheology reveals microscale viscosity gradients in planktonic systems.

Microrheology reveals microscale viscosity gradients in planktonic systems (2020)
Preprint / Working Paper
Guadayol, Ò., Mendonca, T., Segura-Noguera, M., Wright, A. J., Tassieri, M., & Humphries, S. Microrheology reveals microscale viscosity gradients in planktonic systems

Microbial activity in planktonic systems creates a dynamic and heterogeneous microscale seascape that harbours a diverse community of microorganisms and ecological interactions of global significance. In recent decades a great effort has been put int... Read More about Microrheology reveals microscale viscosity gradients in planktonic systems.

Sperm Gatekeeping: 3D Imaging Reveals a Constricted Entrance to Zebra Finch Sperm Storage Tubules (2019)
Journal Article
Mendonca, T., Cadby, A. J., & Hemmings, N. (2019). Sperm Gatekeeping: 3D Imaging Reveals a Constricted Entrance to Zebra Finch Sperm Storage Tubules. Biophysical Journal, 117(11), 2180-2187. https://doi.org/10.1016/j.bpj.2019.10.038

Females across many internally fertilizing taxa store sperm, often in specialized storage organs in their reproductive tracts. In birds, several hundred sperm storage tubules exist in the utero-vaginal junction of the oviduct, and there is growing ev... Read More about Sperm Gatekeeping: 3D Imaging Reveals a Constricted Entrance to Zebra Finch Sperm Storage Tubules.

A trade-off between thickness and length in the zebra finch sperm mid-piece (2018)
Journal Article
Mendonca, T., Birkhead, T. R., Cadby, A. J., Forstmeier, W., & Hemmings, N. (2018). A trade-off between thickness and length in the zebra finch sperm mid-piece. Proceedings of the Royal Society B: Biological Sciences, 285(1883), Article 20180865. https://doi.org/10.1098/rspb.2018.0865

The sperm mid-piece has traditionally been considered to be the engine that powers sperm. Larger mid-pieces have therefore been assumed to provide greater energetic capacity. However, in the zebra finch Taeniopygia guttata, a recent study showed a su... Read More about A trade-off between thickness and length in the zebra finch sperm mid-piece.