Site Saturation Mutagenesis Demonstrates a Central Role for Cysteine 298 as Proton Donor to the Catalytic Site in CaHydA [FeFe]-Hydrogenase

Morra, Simone; Giraudo, Alberto; Di Nardo, Giovanna; King, Paul W.; Gilardi, Gianfranco; Valetti, Francesca

doi:10.1371/journal.pone.0048400

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Site Saturation Mutagenesis Demonstrates a Central Role for Cysteine 298 as Proton Donor to the Catalytic Site in CaHydA [FeFe]-Hydrogenase (2012)
Journal Article
Morra, S., Giraudo, A., Di Nardo, G., King, P. W., Gilardi, G., & Valetti, F. (2012). Site Saturation Mutagenesis Demonstrates a Central Role for Cysteine 298 as Proton Donor to the Catalytic Site in CaHydA [FeFe]-Hydrogenase. PLoS ONE, 7(10), Article e48400. https://doi.org/10.1371/journal.pone.0048400

[FeFe]-hydrogenases reversibly catalyse molecular hydrogen evolution by reduction of two protons. Proton supply to the catalytic site (H-cluster) is essential for enzymatic activity. Cysteine 298 is a highly conserved residue in all [FeFe]-hydrogenas... Read More about Site Saturation Mutagenesis Demonstrates a Central Role for Cysteine 298 as Proton Donor to the Catalytic Site in CaHydA [FeFe]-Hydrogenase.