All Outputs (12)

Pilot Evaluation of S-(3-[18F]Fluoropropyl)-d-Homocysteine and O-(2-[18F]Fluoroethyl)-d-Tyrosine as Bacteria-Specific Radiotracers for PET Imaging of Infection (2024)
Journal Article
Betts, H. M., Luckett, J. C., & Hill, P. J. (2024). Pilot Evaluation of S-(3-[18F]Fluoropropyl)-d-Homocysteine and O-(2-[18F]Fluoroethyl)-d-Tyrosine as Bacteria-Specific Radiotracers for PET Imaging of Infection. Molecular Imaging and Biology, 26, 704-713. https://doi.org/10.1007/s11307-024-01929-7

Purpose There is currently no ideal radiotracer for imaging bacterial infections. Radiolabelled d-amino acids are promising candidates because they are actively incorporated into the peptidoglycan of the bacterial cell wall, a structural feature whic... Read More about Pilot Evaluation of S-(3-[18F]Fluoropropyl)-d-Homocysteine and O-(2-[18F]Fluoroethyl)-d-Tyrosine as Bacteria-Specific Radiotracers for PET Imaging of Infection.

Quorum-sensing, intra- and inter-species competition in the staphylococci (2023)
Journal Article
Williams, P., Hill, P., Bonev, B., & Chan, W. C. (2023). Quorum-sensing, intra- and inter-species competition in the staphylococci. Microbiology, 169(8), https://doi.org/10.1099/mic.0.001381

In Gram-positive bacteria such as Staphylococcus aureus and the coagulase-negative staphylococci (CoNS), the accessory gene regulator (agr) is a highly conserved but polymorphic quorum-sensing system involved in colonization, virulence and biofilm de... Read More about Quorum-sensing, intra- and inter-species competition in the staphylococci.

Conformational analysis and interaction of the Staphylococcus aureus transmembrane peptidase AgrB with its AgrD propeptide substrate (2023)
Journal Article
Bardelang, P., Murray, E. J., Blower, I., Zandomeneghi, S., Goode, A., Hussain, R., …Bonev, B. B. (2023). Conformational analysis and interaction of the Staphylococcus aureus transmembrane peptidase AgrB with its AgrD propeptide substrate. Frontiers in Chemistry, 11, Article 1113885. https://doi.org/10.3389/fchem.2023.1113885

Virulence gene expression in the human pathogen, S. aureus is regulated by the agr (accessory gene regulator) quorum sensing (QS) system which is conserved in diverse Gram-positive bacteria. The agr QS signal molecule is an autoinducing peptide (AIP)... Read More about Conformational analysis and interaction of the Staphylococcus aureus transmembrane peptidase AgrB with its AgrD propeptide substrate.

Enhancing Microbial Electron Transfer through Synthetic Biology and Biohybrid Approaches: Part I: Bioelectrochemistry for sustainable energy conversion (2022)
Journal Article
Myers, B., Hill, P., Rawson, F., & Kovacs, K. (2022). Enhancing Microbial Electron Transfer through Synthetic Biology and Biohybrid Approaches: Part I: Bioelectrochemistry for sustainable energy conversion. Johnson Matthey Technology Review, 66(4), 443-454. https://doi.org/10.1595/205651322X16548607638938

Traditional microbial synthesis of chemicals and fuels often rely on energy-rich feedstocks such as glucose, raising ethical concerns as they are directly competing with the food supply. Therefore, it is imperative to develop novel processes that rel... Read More about Enhancing Microbial Electron Transfer through Synthetic Biology and Biohybrid Approaches: Part I: Bioelectrochemistry for sustainable energy conversion.

Oxygen-Tolerant RAFT Polymerization Initiated by Living Bacteria (2022)
Journal Article
Bennett, M. R., Moloney, C., Catrambone, F., Turco, F., Myers, B., Kovacs, K., …Gurnani, P. (2022). Oxygen-Tolerant RAFT Polymerization Initiated by Living Bacteria. ACS Macro Letters, 11(8), 954-960. https://doi.org/10.1021/acsmacrolett.2c00372

Living organisms can synthesize a wide range of macromolecules from a small set of natural building blocks, yet there is potential for even greater materials diversity by exploiting biochemical processes to convert unnatural feedstocks into new abiot... Read More about Oxygen-Tolerant RAFT Polymerization Initiated by Living Bacteria.

Marine-Source Quorum Quenching Enzyme YtnP to Improve Hygiene Quality in Dental Units (2021)
Journal Article
Sun, X., Hill, P., Liu, J., Qian, J., Ma, Y., & Zhou, S. (2021). Marine-Source Quorum Quenching Enzyme YtnP to Improve Hygiene Quality in Dental Units. Marine Drugs, 19(4), Article 225. https://doi.org/10.3390/md19040225

Biofilm in dental unit water lines may pose a health risk to patients and dental practitioners. An AdiC-like quorum quenching enzyme, YtnP, was cloned from a deep-sea probiotic Bacillus velezensis, and heterologously expressed in E. coli to examine t... Read More about Marine-Source Quorum Quenching Enzyme YtnP to Improve Hygiene Quality in Dental Units.

Gaussia luciferase as a reporter for quorum sensing in staphylococcus aureus (2020)
Journal Article
Blower, I., Tong, C., Sun, X., Murray, E., Luckett, J., Chan, W., …Hill, P. (2020). Gaussia luciferase as a reporter for quorum sensing in staphylococcus aureus. Sensors, 20(15), 1-12. https://doi.org/10.3390/s20154305

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Gaussia luciferase (GLuc) is a secreted protein with significant potential for use as a reporter of gene expression in bacterial pathogenicity studies. To date there are relatively few example... Read More about Gaussia luciferase as a reporter for quorum sensing in staphylococcus aureus.

Fusion expression of bifunctional enzyme complex for luciferin-recycling to enhance the luminescence imaging (2018)
Journal Article
Sun, X., Tang, X., Wang, Q., Chen, P., Hill, P., Fang, B., & Xu, C. (2018). Fusion expression of bifunctional enzyme complex for luciferin-recycling to enhance the luminescence imaging. Journal of Photochemistry and Photobiology B: Biology, 185, 66-72. https://doi.org/10.1016/j.jphotobiol.2018.05.024

Firefly luciferase (Fluc) has been widely used as a bioluminescent monitor. The ATP linear correlation and exogenous luciferin requirement make it useful in most of current imaging systems. However, the utility of this reporter was still limited by t... Read More about Fusion expression of bifunctional enzyme complex for luciferin-recycling to enhance the luminescence imaging.

Ultrasound-mediation of self-illuminating reporters improves imaging resolution in optically scattering media (2018)
Journal Article
Ahmad, J., Jayet, B., Hill, P. J., Mather, M. L., Dehghani, H., & Morgan, S. P. (in press). Ultrasound-mediation of self-illuminating reporters improves imaging resolution in optically scattering media. Biomedical Optics Express, 9(4), https://doi.org/10.1364/BOE.9.001664

In vivo imaging of self-illuminating bio-and chemiluminescent reporters is used to observe the physiology of small animals. However, strong light scattering by biological tissues results in poor spatial resolution of the optical imaging, which also d... Read More about Ultrasound-mediation of self-illuminating reporters improves imaging resolution in optically scattering media.

Correction: Hypothalamic over-expression of VGF in the Siberian hamster increases energy expenditure and reduces body weight gain (PLoS ONE 12:2: (e0172724) DOI: 10.1371/journal.pone.0172724) (2017)
Journal Article
Lewis, J. E., Brameld, J. M., Hill, P., Cocco, C., Noli, B., Ferri, G. L., …Jethwa, P. H. (2017). Correction: Hypothalamic over-expression of VGF in the Siberian hamster increases energy expenditure and reduces body weight gain (PLoS ONE 12:2: (e0172724) DOI: 10.1371/journal.pone.0172724). PLoS ONE, 12(7), https://doi.org/10.1371/journal.pone.0182594

© 2017 Lewis et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are cred... Read More about Correction: Hypothalamic over-expression of VGF in the Siberian hamster increases energy expenditure and reduces body weight gain (PLoS ONE 12:2: (e0172724) DOI: 10.1371/journal.pone.0172724).

Synthesis, in vitro evaluation, and radiolabeling of fluorinated puromycin analogues: potential candidates for PET imaging of protein synthesis (2016)
Journal Article
Betts, H. M., Sephton, S. M., Tong, C., Awais, R., Hill, P. J., Perkins, A. C., & Aigbirhio, F. I. (2016). Synthesis, in vitro evaluation, and radiolabeling of fluorinated puromycin analogues: potential candidates for PET imaging of protein synthesis. Journal of Medicinal Chemistry, 59(20), 9422-9430. https://doi.org/10.1021/acs.jmedchem.6b00968

There is currently no ideal radiotracer for imaging protein synthesis rate (PSR) by positron emission tomography (PET). Existing fluorine-18 labelled amino acid-based radiotracers predominantly visualize amino acid transporter processes, and in many... Read More about Synthesis, in vitro evaluation, and radiolabeling of fluorinated puromycin analogues: potential candidates for PET imaging of protein synthesis.

The dynamic balance of import and export of zinc in Escherichia coli suggests a heterogeneous population response to stress (2015)
Journal Article
Takahashi, H., Oshima, T., Hobman, J. L., Doherty, N., Clayton, S. R., Iqbal, M., …Stekel, D. J. (2015). The dynamic balance of import and export of zinc in Escherichia coli suggests a heterogeneous population response to stress. Interface, 12(106), Article 20150069. https://doi.org/10.1098/rsif.2015.0069

Zinc is essential for life, but toxic in excess. Thus all cells must control their internal zinc concentration. We used a systems approach, alternating rounds of experiments and models, to further elucidate the zinc control systems in Escherichia col... Read More about The dynamic balance of import and export of zinc in Escherichia coli suggests a heterogeneous population response to stress.