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All Outputs (5)

Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells (2015)
Journal Article
Sidney, L. E., Branch, M. J., Dua, H. S., & Hopkinson, A. (2015). Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells. Cytotherapy, 17(12), https://doi.org/10.1016/j.jcyt.2015.08.003

Background: The limbal area of the corneal stroma has been identified as a source of mesenchymal-like stem cells, which have potential for exploitation as a cell therapy. However, the optimal culture conditions are disputed and few direct media compa... Read More about Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells.

Phenotypic change and induction of cytokeratin expression during in vitro culture of corneal stromal cells (2015)
Journal Article
Sidney, L. E., McIntosh, O. D., & Hopkinson, A. (2015). Phenotypic change and induction of cytokeratin expression during in vitro culture of corneal stromal cells. Investigative Ophthalmology & Visual Science, 56(12), https://doi.org/10.1167/iovs.15-17810

Purpose: Cells of the corneal epithelium and stroma can be distinguished in vivo by different intermediate filaments, cytokeratins for corneal epithelial cells (CEC) and vimentin for keratocytes. Isolated and cultured keratocytes change phenotype, lo... Read More about Phenotypic change and induction of cytokeratin expression during in vitro culture of corneal stromal cells.

Corneal Decellularization: A Method of Recycling Unsuitable Donor Tissue for Clinical Translation? (2015)
Journal Article
Wilson, S. L., Sidney, L. E., Dunphy, S. E., Dua, H. S., & Hopkinson, A. (2016). Corneal Decellularization: A Method of Recycling Unsuitable Donor Tissue for Clinical Translation?. Current Eye Research, 41(6), 769-782. https://doi.org/10.3109/02713683.2015.1062114

Background: There is a clinical need for biomimetic corneas that are as effective, preferably superior, to cadaveric donor tissue. Decellularized tissues are advantageous compared to synthetic or semi-synthetic engineered tissues in that the native m... Read More about Corneal Decellularization: A Method of Recycling Unsuitable Donor Tissue for Clinical Translation?.

Endothelial cell loss following tissue harvesting by pneumodissection for endothelial keratoplasty: an ex vivo study. (2015)
Journal Article
SL, A., A, G., LE, S., MS, E., A, A., & HS, D. (2015). Endothelial cell loss following tissue harvesting by pneumodissection for endothelial keratoplasty: an ex vivo study. British Journal of Ophthalmology, 99(5), 710-713. https://doi.org/10.1136/bjophthalmol-2014-306560

Aims
To study ex vivo the difference in endothelial cell density (ECD) in tissue harvested by pneumodissection for pre-Descemet's endothelial keratoplasty (PDEK) and Descemet's membrane endothelial keratoplasty (DMEK).

Methods
Tissue for PDEK... Read More about Endothelial cell loss following tissue harvesting by pneumodissection for endothelial keratoplasty: an ex vivo study..