All Outputs (2)

Next-Generation Phage Display to Identify Peptide Ligands of Deubiquitinases (2022)
Book Chapter
Spiliotopoulos, A., Maurer, S. K., Tsoumpeli, M. T., Bonfante, J. A. F., Owen, J. P., Gough, K. C., & Dreveny, I. (2023). Next-Generation Phage Display to Identify Peptide Ligands of Deubiquitinases. In J. Maupin-Furlow, & M. J. Edelmann (Eds.), Deubiquitinases: Methods and Protocols (189-218). Humana Press. https://doi.org/10.1007/978-1-0716-2803-4_12

Phage display (PD) is a powerful method and has been extensively used to generate monoclonal antibodies and identify epitopes, mimotopes, and protein interactions. More recently, the combination of next-generation sequencing (NGS) with PD (NGPD) has... Read More about Next-Generation Phage Display to Identify Peptide Ligands of Deubiquitinases.

A Simple Whole-Plasmid PCR Method to Construct High-Diversity Synthetic Phage Display Libraries (2022)
Journal Article
Tsoumpeli, M. T., Gray, A., Parsons, A. L., Spiliotopoulos, A., Owen, J. P., Bishop, K., …Gough, K. C. (2022). A Simple Whole-Plasmid PCR Method to Construct High-Diversity Synthetic Phage Display Libraries. Molecular Biotechnology, 64, 791-803. https://doi.org/10.1007/s12033-021-00442-4

Phage display technology utilises peptide and antibody libraries with very high diversities to select ligands with specific binding properties. The production of such libraries can be labour intensive and technically challenging and whilst there are... Read More about A Simple Whole-Plasmid PCR Method to Construct High-Diversity Synthetic Phage Display Libraries.