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All Outputs (5)

A single-cell atlas of pig gastrulation as a resource for comparative embryology (2024)
Journal Article
Simpson, L., Strange, A., Klisch, D., Kraunsoe, S., Azami, T., Goszczynski, D., …Alberio, R. (2024). A single-cell atlas of pig gastrulation as a resource for comparative embryology. Nature Communications, 15(1), Article 5210. https://doi.org/10.1038/s41467-024-49407-6

Cell-fate decisions during mammalian gastrulation are poorly understood outside of rodent embryos. The embryonic disc of pig embryos mirrors humans, making them a useful proxy for studying gastrulation. Here we present a single-cell transcriptomic at... Read More about A single-cell atlas of pig gastrulation as a resource for comparative embryology.

Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species (2021)
Journal Article
Kinoshita, M., Kobayashi, T., Planells, B., Klisch, D., Spindlow, D., Masaki, H., …Smith, A. (2021). Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species. Development, 148(23), Article dev199901. https://doi.org/10.1242/dev.199901

Despite four decades of effort, robust propagation of pluripotent stem cells from livestock animals remains challenging. The requirements for self-renewal are unclear and the relationship of cultured stem cells to pluripotent cells resident in the em... Read More about Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species.

Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system (2018)
Journal Article
Canizo, J. R., Vazquez Echegaray, C., Klisch, D., Aller, J. F., Paz, D. A., Alberio, R. H., …Guberman, A. S. (2018). Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system. BMC Research Notes, 11, Article 509. https://doi.org/10.1186/s13104-018-3627-8

Objectives The use of induced pluripotent stem (iPS) cells as an alternative to embryonic stem cells to produce transgenic animals requires the development of a biotechnological platform for their generation. In this study, different strategies fo... Read More about Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system.

Transcription factor MYB26 is key to spatial specificity in anther secondary thickening formation (2017)
Journal Article
Yang, C., Song, J., Ferguson, A. C., Klisch, D., Simpson, K., Mo, R., …Wilson, Z. A. (2017). Transcription factor MYB26 is key to spatial specificity in anther secondary thickening formation. Plant Physiology, 175(1), https://doi.org/10.1104/pp.17.00719

Successful fertilization relies on the production and effective release of viable pollen. Failure of anther opening (dehiscence), results in male sterility, although the pollen may be fully functional. MYB26 regulates the formation of secondary thick... Read More about Transcription factor MYB26 is key to spatial specificity in anther secondary thickening formation.