Identifying new biomarkers of aggressive Group 3 and SHH medulloblastoma using 3D hydrogel models, single cell RNA sequencing and 3D OrbiSIMS imaging

Linke, Franziska; Johnson, James E. C.; Kern, Stefanie; Bennett, Christopher D.; Lourdusamy, Anbarasu; Lea, Daniel; Clifford, Steven C.; Merry, Catherine L. R.; Stolnik, Snow; Alexander, Morgan R.; Peet, Andrew C.; Scurr, David J.; Griffiths, Rian L.; Grabowska, Anna M.; Kerr, Ian D.; Coyle, Beth

doi:10.1186/s40478-022-01496-4

All Outputs (5)

Application of a 3D hydrogel-based model to replace use of animals for passaging patient-derived xenografts (2023)
Journal Article
Jones, S., Ashworth, J. C., Meakin, M., Collier, P., Probert, C., Ritchie, A. A., Merry, C. L. R., & Grabowska, A. M. (2023). Application of a 3D hydrogel-based model to replace use of animals for passaging patient-derived xenografts. In Vitro Models, 2(3-4), 99-111. https://doi.org/10.1007/s44164-023-00048-x

Purpose
This 3D in vitro cancer model for propagation of patient-derived cells, using a synthetic self-assembling peptide gel, allows the formation of a fully characterised, tailorable tumour microenvironment. Unlike many existing 3D cancer models,... Read More about Application of a 3D hydrogel-based model to replace use of animals for passaging patient-derived xenografts.

OptoRheo: Simultaneous in situ micro-mechanical sensing and imaging of live 3D biological systems (2023)
Journal Article
Mendonca, T., Lis-Slimak, K., Matheson, A. B., Smith, M. G., Anane-Adjei, A. B., Ashworth, J. C., …Wright, A. J. (2023). OptoRheo: Simultaneous in situ micro-mechanical sensing and imaging of live 3D biological systems. Communications Biology, 6, Article 463. https://doi.org/10.1038/s42003-023-04780-8

Biomechanical cues from the extracellular matrix (ECM) are essential for directing many cellular processes, from normal development and repair, to disease progression. To better understand cell-matrix interactions, we have developed a new instrument... Read More about OptoRheo: Simultaneous in situ micro-mechanical sensing and imaging of live 3D biological systems.

Hydrogel-Based Pre-Clinical Evaluation of Repurposed FDA-Approved Drugs for AML (2023)
Journal Article
James, J. R., Curd, J., Ashworth, J. C., Abuhantash, M., Grundy, M., Seedhouse, C. H., …Thompson, A. (2023). Hydrogel-Based Pre-Clinical Evaluation of Repurposed FDA-Approved Drugs for AML. International Journal of Molecular Sciences, 24(4), Article 4235. https://doi.org/10.3390/ijms24044235

In vivo models of acute myeloid leukemia (AML) are low throughput, and standard liquid culture models fail to recapitulate the mechanical and biochemical properties of the extracellular matrix-rich protective bone marrow niche that contributes to dru... Read More about Hydrogel-Based Pre-Clinical Evaluation of Repurposed FDA-Approved Drugs for AML.

OptoRheo: Simultaneous in situ micro-mechanical sensing and 3D imaging of live cell cultures (2023)
Preprint / Working Paper
Mendonca, T., Lis-Slimak, K., Matheson, A. B., Smith, M. G., Anane-Adjei, A. B., Cavanagh, R., …Wright, A. J. OptoRheo: Simultaneous in situ micro-mechanical sensing and 3D imaging of live cell cultures

Biomechanical cues from the extracellular matrix (ECM) are essential for directing many cellular processes, from normal development and repair, to disease progression. To better understand cell-matrix interactions, we have developed a new instrument... Read More about OptoRheo: Simultaneous in situ micro-mechanical sensing and 3D imaging of live cell cultures.

Identifying new biomarkers of aggressive Group 3 and SHH medulloblastoma using 3D hydrogel models, single cell RNA sequencing and 3D OrbiSIMS imaging (2023)
Journal Article
Linke, F., Johnson, J. E. C., Kern, S., Bennett, C. D., Lourdusamy, A., Lea, D., …Coyle, B. (2023). Identifying new biomarkers of aggressive Group 3 and SHH medulloblastoma using 3D hydrogel models, single cell RNA sequencing and 3D OrbiSIMS imaging. Acta Neuropathologica Communications, 11(1), Article 6. https://doi.org/10.1186/s40478-022-01496-4

The most common malignant brain tumour in children, medulloblastoma (MB), is subdivided into four clinically relevant molecular subgroups, although targeted therapy options informed by understanding of different cellular features are lacking. Here, b... Read More about Identifying new biomarkers of aggressive Group 3 and SHH medulloblastoma using 3D hydrogel models, single cell RNA sequencing and 3D OrbiSIMS imaging.